汞中毒大鼠脊髓GFAP表达与药物干预研究_医学论文.docVIP

汞中毒大鼠脊髓GFAP表达与药物干预研究_医学论文 汞中毒大鼠脊髓GFAP表达与药物干预研究_医学论文 作者：侯殿东，施丽飞，张韵，孙卫民，滕文锋，刘辉 【关键词】 汞中毒；神经病理性疼痛；脊髓；,星形胶质细胞；胶质纤维酸性蛋白 　　摘要： 目的 观察HgCl2对大鼠脊髓星形胶质细胞的影响。方法 SD大鼠30只,雌雄各半，体重160～200g。随机分为3组，每组10只。大剂量组按17mg/kg（1/4 LD50）经口灌胃HgCl2溶液，1次/d。小剂量组按85mg/kg（1/8 LD50）经口灌胃HgCl2溶液，1次/d。对照组经口灌胃生理盐水2ml，1次/d，3组均连续灌胃（20±2）d。染汞组建成亚急性汞中毒模型后，各组均处死5只雄鼠，留5只雌鼠作药物干预试验。染汞组用二巯丙磺钠注射液（DMPS）按28mg/kg腹腔注射驱汞2个疗程，小剂量组仅用DMPS,大剂量组在用DMPS的期间内，按2922/(kg#12539d)经口灌胃己酮可可碱（ POF）溶液14d。实验结束后处死全部动物，灌注固定取出脊髓L5-6节并制成病理切片。用免疫组化链酶亲和素-生物素-过氧化物酶聚合物（SABC）法测定脊髓胶质纤维酸性蛋白(GFAP)。结果 大、小剂量组GFAP平均灰度均显著低于对照组,阳性细胞率均显著高于对照组。DMPS+POF显著降低GFAP阳性细胞率，提高平均灰度值，单纯应用DMPS无此作用。结论 亚急性HgCl2中毒大鼠脊髓GFAP表达上调，POF能够有效抑制星形胶质细胞激活。 　　关键词：汞中毒；神经病理性疼痛；脊髓； 星形胶质细胞；胶质纤维酸性蛋白 　　Study on spinal cord GFAP of mecury poisoning rat and drug trial 　　Abstract： Objective To observe the effect of HgCl2 on the astrocyte in spinal cord.Methods Thirty SD rats(15♀,15♂,weighting 160～200g) were randomized into 3 groups of 10(5♀,5♂)each. Group A and group B received HgCl2 juice at a dose of 17mg/kg(1/4 LD50),8.5 mg/kg(1/8 LD50) respectively by gavage daily . Group C received 0.9% saline 2 ml by gavage daily.To found the sub-acute mercury poisoning rat model within(20±2) days.Then male rats in each group were dissected and the chelator( DMPS) was injected into the abdomen of female rats of group A and B at a dose of 28 mg/kg for 2 periods .Group A received DMPS+POF(pentoxifylline) and group B received DMPS only.The POF was used at a dose of 29.22 mg/kg by gavage daily for 14 days .The rats were all dissected and the spinal cord (L5-6) were gotten after the trial finished.Then the pathological section of spinal cord was made from and GFAP was detected .Results The average gray scale in group A and group B are all less and the positive cell rate are all larger significantly compaired with control group.The POF depress the activation of astrocyte significantly.Conclusion Sub-acute poisoning of HgCl2 enhance GFAP level significantly in spinal cord,and POF depresses the activation of