活血熄风方对局灶性脑缺血大鼠血管内皮生长因子mRNA表达的影响_药学论文.docVIP

活血熄风方对局灶性脑缺血大鼠血管内皮生长因子mRNA表达的影响_药学论文 活血熄风方对局灶性脑缺血大鼠血管内皮生长因子mRNA表达的影响_药学论文 【摘要】 目的观察活血熄风方对局灶性脑缺血模型大鼠脑梗塞体积及脑组织中血管内皮生长因子（VEGF mRNA）表达的影响。方法Wistar雄性大鼠随机分为假手术组，模型组，活血熄风方高、中、低3个剂量组（剂量分别为20，10，5 g·kg-1）和尼莫地平对照组（0.02 g·kg-1），采用光化学法建立局灶性脑缺血大鼠模型，造模前7d灌胃给药，造模后24 h 取材，TTC染色法测脑梗塞体积，逆转录聚合酶链式反应（RT-PCR）法测VEGF mRNA表达。结果 与模型组比较，活血熄风方各剂量组的脑梗塞体积明显减少（P＜0.01），VEGF mRNA表达明显升高 (P＜0.01)。结论活血熄风方可明显减少局灶性脑缺血大鼠脑梗塞体积，具有脑保护作用，其作用机制可能与增强VEGF mRNA表达有关。 【关键词】 活血熄风方；光化学法；血管内皮生长因子mRNA； 逆转录聚合酶链式反应； 局灶性脑缺血 ChinaAbstract:ObjectiveTo observe the effects of Huoxue Xifeng Recipe (HXR) on infarction volume and the expressions of VEGF mRNA in brain tissue after focal cerebral infarction(FCI) in rats.MethodsWistar male rats were randomly divided into six groups: sham group, model group, HXR treated group(20，10，5 g·kg-1 as high，middle and low dose respectively) and nimodipine control group(0.02 g·kg-1). The model of FCI was established based on the principle of photo-chemistry initiation of thrombosis, intragastric administration continued 7 days before modeling and draw the material after 24h. Infarction volume was detected by TTC staining technique, the expression of VEGF mRNA was detected by reverse transcription polymerase chain reaction(RT-PCR). ResultsThe infarction volume was decreased and the expressions of VEGF mRNA was higher in HXR trerted groups than that of FCI model rats (P＜0.05 or P＜0.01).ConclusionHXR can decreased infarction volume and has a protective effect on FCI rats, and the mechanism may be related to increasing the expression of VEGF mRNA. 　　Key words:Huoxue Xifeng Recipe; Photo-chemistry; VEGF mRNA; RT-PCR; Focal cerebral infarction 　　脑缺血后，由于脑组织缺血、缺氧，缺血中心区细胞迅速死亡，半暗区神经细胞处于低氧、低灌注状态，及时恢复该区域血流供应有望改善患者的神经功能。而半暗区血流的恢复有赖于侧枝循环的建立，血管再生是决定这些细胞存活的关键因素，与缺血性脑卒中病人的预后关系密切［1］。在血管再生过程中，众多因子参与调节，血管内皮生长因子(vascular endothelial growth factor,VEGF) 是已知作用最强和特异性最高的促血管生长因子［2］，在血管生成和神经保护方面发挥着重要作用。 　　活血熄风方是在“淤血生风”假说［3］基础上提出的治疗中风的新组方，全方以活血化淤为主，通过活血化淤以求逐淤熄风，经临床验证疗效满意，已有实验证实活血熄风方具有脑保护作用［4］。本实验用TTC染色法观察光化学法诱导的局