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一个水稻OsWNK基因的分离及表达分析.pdf
Guihaia 29(3) :404- 407 2009 5
Os WNK
1 1 2 1*
王小兰 , 刘顺枝 , 何光存, 田长恩
( 1. , 510006;
2. , 430072 )
: cDNA A t WN K 1EST
( GenBank : BU572310) ,EST , cDNA Os WN K
cDNA, 677 ,
( GenBank : D 837532)3 Northern , ,
,.
: ; Os WN K ; cDNA ;
: 966, 786 : A : 2009
* Isolation and expression analysis
of an Os WNK gene in rice
1 1 2 1*
WANG XiaoLan , LIU ShunZhi , HE GuangCun , TIAN ChangEn
( 1.Guangzhou K ey Laboratory f or Functional Studies on Plant StressResistant Genes , School of L if e Sciences ,
Guangzhou Uni ersity , Guangzhou, 510006, China; 2.Key Laboratory of the Ministry of Educationf or Plant
De elop mental B iology , College of L if e Sciences, Wuhan Uni ersity , Wuhan430072, China )
Abstract: BPHiw008,an EST ( expressed sequence tag) which is highly homologouswith A rabidop sis WN K 1(with
no lysine kinase), has been screeded from the rice cDNA SSH library. Using this EST as probe to screen the cDNA
library induced by brown planthopper sucking, a full length of cDNA with 2. 4 Kb in size was isolated and named as
Os WNK . Os WNK is deduced to encodea 677aminoacid protein OsWNK through BLAST . Only 3 aminoacid resi
dues of Os WN K are different from those of A t WN K 1. Result of RNA gel blot analysis showed that Os WN K was
upregulated in brown planthoppersucked rice, indicating that Os WNK may play a role in resistance to brown plan
thopper of rice.
Key words: brown planthopper; Os WNK ; cDNA screening; rice
( brown planthopper) , ,
, ( ,
1999) ,
, ,
, , ( Hanks,
2~ 3
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