微生物合成的聚羟基链烷酸在细胞内的积聚及提取.pdfVIP

高分子材料科学与工程990150 高分子材料科学与 工程 POLYMER MATERIALS SCIENCE ENGINEERING 1999年 第1期 No.1 1999 微生物合成的聚羟基链烷酸在细胞内的积聚及提取 苏　涛 (广西大学化学系,南宁,530004) 石海平 (广西卫生管理干部学院药学系,南宁,530021) 　　摘要　综述了以聚β-羟基丁酸(PHB)为代表的可用作可降解塑料的聚羟基链烷酸 (PHA)在细胞内积聚的形态、动力学及其提取技术。 　　关键词　可降解塑料,聚羟基丁酸,聚羟基链烷酸 ACCUMULATION IN CELLS AND EXTRACTION OF POLY (HYDROXYALKANOIC ACIDS) SYNTHESIZED BY MICROORGANISMS Su Tao (Depart. Chemistry. Guangxi University, Nanning) Shi Haiping (Depart. Pharmacy Guangxi College of Sanitary Administration Nanning) 　　ABSTRACT　With 25 references, this review discussed a kind of degradable plastics synthesized by microorganisms, polyhydroxyalkanoic acids(PHA), representatively polyhydroxybutyric acid(PHB), mainly about its existing states in the cells, accumulation kinetics, and its extraction from the cells. PHB existed in bacteria cells as tiny granules, which were in the form of a mobile amorphous elastomer and could coalesce during cell growth. After PHA synthesis has been induced by a nutrient limitation, its rate droped from the maximum value in zero, analogous to the monomolecular decay model. PHA was extracted from the cells by a partially halogenated hydrocarbon (solvent) such as dichloromethane and precipitated by addition of an organic liquid(non-solvent) which was not able to desolve PHA. After use, the solvent and non-solvent should be recoved, seperated and re-used. 　　Keywords　degradable plastics, polyhydroxyalkanoic acids(PHA