微酸环境响应的聚天冬氨酸修饰脂质体的制备与表征.pdfVIP

第66 卷 第3 期 化 工 学 报 Vol.66 No.3 March 2015 2015 年3 月 CIESC Journal ·1234 · 微酸环境响应的聚天冬氨酸修饰脂质体的制备与表征 王丽琳，沈骧一，苏海佳，曹辉 （北京化工大学北京市生物加工过程重点实验室，北京 100029 ） 摘要：利用带负电的聚天冬氨酸分子与部分带正电的磷脂双分子层之间的静电吸附作用，通过“一步法”成功制 备了聚天冬氨酸（PASP ）修饰脂质体（PLPs ），实现了修饰脂质体微酸环境响应性。将脂质体制备与PASP 修饰 同时完成的“一步法”大大简化了制备工艺，提高了脂质体（PLPs ）的制备效率。通过单因素筛选确定了pH 敏 感性显著的修饰脂质体制备条件，即外水相溶液PASP 浓度2.5% （质量分数），pH8.5 。透射电子显微镜照片显示 PLPs 由于修饰剂的存在具有更大的粒径，且表面电负性高，证明了“一步法”成功制备了pH 敏感性修饰脂质体。 关键词：pH 敏感；聚天冬氨酸；脂质体；制备；纳米粒子 DOI ：10.11949/j.issn.0438-1157 中图分类号：R 944.1 文献标志码：A 文章编号：0438—1157 （2015）03—1234—06 Preparation and characterization of subacid environment responsive polyaspartic acid modified liposomes WANG Lilin，SHEN Xiangyi，SU Haijia，CAO Hui (Beijing Key Laboratory of Bioprocess，Beijing University of Chemical Technology，Beijing 100029，China) Abstract ：PASP modified liposomes (PLPs) were prepared by using negatively charged PASP chains linked to the head of positively charged phospholipid via electrostatic adsorption. A “one-step” method was developed to form and modify the liposomes. Single factor experiments were conducted to prepare high pH sensitive PLPs. The optimal formula in preparing PLPs was 2.5%(mass) PASP solution with pH8.5. TEM results showed that PASP modified liposomes had a larger particle size and their surface with higher electro-negativity. PASP modified liposomes are a kind of relatively stable emulsion system. Key words：pH sensitivity；polyaspartic acid ；liposomes ；preparation ；nanopa