湖北恩施地区烟草青枯菌的生理分化研究.pdfVIP

2014-1035 5 ChineseTobacco Science 79 1 1,2 1 1 2 1* 1. 430070 2. 430030 Ralstonia solanacearum 8 54 6 13 3 54 ⅢPCR 54 280 bp 144 bp Ⅰ S572.08 1007-5119201405-0079-05 DOI 10.13496/j.issn.1007-5119.2014.05.015 Physiological Specialization of Ralstonia solanacearum Strains Isolated from Enshi, Hubei Province 1 1,2 1 1 2 1* LIU Hailong , LI Yanyan , YAN Yaqin , ZHENG Lu , LI Xihong , HUANG Junbin (1.The Key Laboratory of Plant Pathology of Hubei Province, College of Plant Science and Technology, Huazhong Agricultural University, Wuhan 430070, China; 2.Tobacco Research Institute of Hubei Province, Wuhan 430030, China) Abstract: To provide theoretical information for breeding and utilizing bacterial wilt-resistant tobacco cultivars race, biovar and phylotype of Ralstonia solanacearum of tobacco in Hubei province were identified. Diseased plant samples of tobacco bacterial wilt were collected from eight counties of Enshi area in Hubei province, and 54 strains of the pathogen, Ralstonia solanacearum, were isolated from these samples by using the dilution plate isolation method and identified by specific-PCR assay. Based on reactions on six physiological-race hosts, all 54 strains were proved to belong to race 1. On the basis of ability for oxidizing three hexose (lactose， maltose and cellobiose) and three disaccharides (mannitol, sorbitol and dulcite), all strains were identified as biovar III. Phylotype specific multiplex PCR revealved that all