生姜多酚的优化提取及其抗氧化性研究.pdfVIP

, , , (徐州工程学院食品工程学院, 江苏徐州221008) : 以生姜为原料, 有机溶剂浸提法提取生姜多酚通过单因素 正交实验探讨了溶剂种类溶剂浓度料液比 提取时间提取温度以及提取次数等对生姜多酚提取率的影响结果表明: 鲜生姜 60e 以下干燥, 粉碎过 60目筛, 最 佳溶剂75% 乙醇, 料液比 1B25, 70e 条件下回流提取 2次, 每次 215h, 生姜多酚提取率最高达到 1179% 抗氧化实验表 明, 生姜多酚具有一定的清除 DPPH 自由基的能力, 且清除能力与浓度呈较明显的量效关系, 气相色谱-质谱联用 ( GC-MS)分析鉴定出 35种化合物, 其中多酚类化合物 7种, 相对含量达 45102% , 表明了生姜的乙醇提取物中富含多 酚类抗氧化活性成分 : 生姜多酚, 提取,优化, 工艺研究, 抗氧化性 Study on extraction and antioxidation of ginger polyphenols TANG Sh i- ron g, L I Chao, SONG H ui, XU Jin ( School of Food E gi eeri g, Xuzhou I stitute ofTech ology, Xuzhou 221008, Ch i a) Abstrac:t Extrac tio tech o logy o f g i g er p olyp he o ls i g i g er w as s tud ied w ith so lve t extrac toi 1Effec ts o f so vl e t spec ies, e tha o l co ce tratio , ra tio o f m a te ria l to liqu id, extrac toi tmi e, extrac toi tem pe ratu re a d extractio tmi es o extrac tio ra te o fg i g e rp o yl p he o ls w e re i vestgi a ted by s i g le fac to r a d o rthogo a l test1The optmi um extrac toi co d itoi s fo r g i g e r po lyp he o ls w e re id e tified as the fo llow s: the pa rtic le size 60 scree m esh, 75% etha o l as so lve ,t ra tio o fm a te ria l to liqu id 1B25, ex tractio tempe ra tu re 70e , extrac tio tmi e 215h w ith tw o tmi es a fte r dry i g less tha 60e o f fresh g i ge r1The extrac toi ra toi o fg i g er po lyp he o lsw as up to 1179% 1The exp ermi e t of a tioxid a t activity show ed tha t g i ge r po lyp he o ls had g ood ac tiv ity o f scave g i g DPPH rad ica ls, a d the scave g i g activity had obvious dose- effec t re la toi sh ip o f co ce tra toi 1A d the extractw as a a lyzed by GC- MS 17 comp ou d s m gi ht be lo g ed to p o yl p he o ls i the 35 com pou d s id e tified by GC- MS a a lysis, a d re la