铅_Pb_胁迫对黄菖蒲叶片生理生化指标的影响_黄苏珍.pdfVIP

, Jour al of A hui Agri.Sci.2008, 36(25):10760-10762　　　　　　　　　　　　　　　　　　　姜丽　　张士敏 (Pb) 黄苏珍　(, 330032) 　[目的]研究铅(Pb)胁迫对黄菖蒲叶片生理生化指标的影响。[方法] 以黄菖蒲(Iris pseudacorus L.)为材料, 采用沙 方法, 对不同 Pb 浓度(0、2、4、6、8、10 mmol/L)和处理时间(7、14、21、28 d)胁迫下植物的叶片叶绿素、丙二醛(MDA)和脯氨酸(Pro)含量以及超氧化物歧 化酶(SOD)活性的变化进行了研究。[结果]结果表明:在Pb 胁迫下, 黄菖蒲植物叶绿素含量随Pb 处理浓度的增加而降低, 而随时间的 延长相同Pb 处理下叶绿素的含量先降后升。在6 mmol/L 以上浓度Pb 胁迫下, 黄菖蒲叶片的MDA 水平明显高于对照;SOD 活性和脯氨 酸含量在Pb 胁迫下均有不同程度增加。脯氨酸在Pb 胁迫下出现相对更大幅度的上升, 可能是使黄菖蒲提高诱导胁迫抗性的主要调节 物质之一。[结论] 初步揭示了不同浓度Pb 胁迫对黄菖蒲生理生化代谢的影响, 为进一步探讨Pb 胁迫下黄菖蒲生理生化指标与Pb 耐 性能力的相关关系奠定了基础。 　黄菖蒲;铅(Pb)胁迫;生理生化指标 　S 311　　　A　　　0517-6611(2008)25-10760-03 Effectsof Lead(Pb)Stresson the Physiological and Biochemical Indices of Iris p seudacorus L. HUANG Su-zhen　(College of Resource E viro me t Ma ageme t, Jia gxi U iversity of Fi a ce Eco omy, Na cha g, Jia gxi 330032) Abstract 　[Objective] The research aimed to study the effects of Pb o the physiological a d biochemical i dices of Irispseudacorus L.[Method] Tak- i g I .pseudacorus L.as material, The cha ges of the co te ts of chlorophylls, MDA, proli e a d the activity of SOD of I .pseudacorus L.leaves were studied with differe t Pb co ce tratio a d differe t treatme t time by sa d culture.[Result] The results showed that the co te ts of chlorophylls i the leaves of I .pseudacorus L.decreased as Pb co ce tratio s were raised, a d the co te ts of chlorophylls dropped a d the slowly raised at same Pb treat- me t as treatme t period lo ger.The co te ts of MDA i the leaves of I .pseudacorus L.treatedwith above6 mmol/LPb were sig ifica tly differe t with those of co trol.The co te ts of proli e a d the activities of SOD i the leaves of I .pseudacorus L.i creased as thePb co ce tratio s were raised.The co te ts of proli e were i creased much higher i the leaves of I .pseudacorus L.u der Pb treatme ts, which showed that proli e might be o e of the most importa t materials for the tolera ce of I .pseudacorus L..[Co clusio ] The study pr