四氯化碳综合法制备大鼠肝硬化模型.pdfVIP

2005 5 22 5 J Cl n R es , M ay 2005, V ol 22, N o5 6 19 论著 叶春华 刘浔阳 ( 中南大学湘雅三医院普外科, 湖南 长沙 4100 13) [ ]目的探讨建立一种理想的大鼠肝硬化模型的复制方法并加以评 , 为下一步探索肝硬化门脉 高压症治疗方法提供可靠稳定的模型动物方法90 只雄性SD 大鼠随机分为两组: 实验组( 70 只) 采用四 氯化碳( CCl4 ) + 苯巴比妥钠+ 食用白酒进行诱导, 诱导期间CCl4 浓度在一定范围内逐步增加, 并用食用白酒 替代医用乙醇溶液; 对照组( 20 只) 给与普通饮水和饲料, 频度及剂量同实验组同时采用一系列的检查方 法加以评估结果实验组70 只大鼠造模结束时, 死亡9 只, 死亡率 12 . 86% 实验结束时, 按照成模标准, 造模成功大鼠共61 只, 均可观察到肝硬化结节及镜下假小叶形成, 成模率 87 . 14% 实验组门静脉压力比对 照组明显增高( P 0. 05) ; 血清总胆红素谷丙转氨酶及谷草转氨酶较正常对照组明显升高( P 0 . 05) , 而 血清白蛋白较之正常对照组明显降低( P 0 . 05) 对照组 20 只全部存活结论该方法可形成稳定的肝 硬化门脉高压症模型, 可用于肝硬化门脉高压症方面的相关研究此模型制作周期短( 仅需9 周) , 成模率高, 死亡率低, 具有简便快捷高效的优点, 适于大批量制作 [ ] 肝硬化, 实验性; 疾病模型, 动物; 大鼠; 四氯化碳 Study on Method of Inducing Hepatic Cirrhosis Model in Rats by Carbon Tetrachloride YE Chunhua, LI U X uny ang ( Dep artment of General Surgery , the T hird X iangy a H osp ital , Central South Uni ers ity , Changsha, 4 10013, China ) [Abstract]Object veT o dev elo p and evaluate an deal means to nduce c r rhot c model n rats for next study o n l ver c r rhos s w th po rtal hypertens on treatment .M ethodsN nety male SD r ats w er e randomly d v ded nto t w o gr oups, T he ex per ment g roup ( n = 70) w as t reated w th CCl , PBS and eatable alcoho l and the 4 co ncentrat on of CCl4 and eatable alcohol w ere ncreased stage by stag e n a cer ta n range dur ng the ex per ment . T he co ntr ol gr oup ( n = 20) w as treated w th common dr nk ng w ater and an mal f eeds, the f requency and do se w ere the same as the exper ment g ro up , meanw h le evaluated by a ser es of check methods .Results After be ng nduced w th abov e means, the rat mort al t y of the ex per ment gr oup w as