有氧运动对肥胖大鼠心肌脂肪酸代谢干预作用的研究.pdfVIP

27 2 V l27 N 2 2010 3 JOURNAL OF XIAN PHYSICAL EDUCAT ION UNIVERSITY Mar2010 : 1001747 ( 2010) : A : G80407 1, 2 2 2 2 2 2 刘远新 , 周 里, 范 鹏, 佟 吉力 , 马 艳 , 孔淑珍 ( 1. , 200438; 2. , 710068) : 目的观察肥胖大鼠心肌型脂肪酸结合 白(H- FABP) 含量的变化, 探索有氧运动对肥胖大鼠心肌脂肪酸代 谢的干预作用方法SD 雄性大鼠给予6 周的高脂饮食建造肥胖模型, 造模成功的大鼠随机分为肥胖对照组和肥胖 运动组, 肥胖运动组进行 12 周的有氧耐力运动后测定大鼠心脏重量指数, 使用铜显色法测定心肌游离脂肪酸( FFA) 浓度, 应用Western- Bl t 方法检测心肌型脂肪酸结合 白含量结果 肥胖对照组大鼠心脏重量指数FFA 浓度较肥 胖运动组明显增加, 差异非常显著( P 0. 01) 肥胖运动组大鼠心脏H- FABP 白含量较对照组明显增加, 差异显 著(P 0. 05) 结论 高脂饮食诱导肥胖大鼠存在左室肥厚, 有氧运动减缓了肥胖大鼠左室肥厚的进展, 使心肌FFA 浓度降低, 可能与H- FABP 白表达增加调节了心肌的脂肪酸代谢有关 : 有氧运动; H- FABP; FFA; 肥胖大鼠; 心肌 Effects of Aerobic Exercise on Myocardial Fatty Acid Metabolism in Obese Rats 1, 2 2 2 2 2 2 LIU Yuanxin , ZHOU Li , FAN Peng ,TONG Jie ,MA Yan , KONG Shuzhen ( 1. Sch l f sp rts science, Shanghai University f Sp rt, Shanghai 200438, China; 2. Xian Physical Educati n University, Xian 710068, China) Abstract:Objective: T bserve the heart- type fatty acid binding pr tein ( H- FABP) c ntent changes, and expl re the effect f aer bic exercise n my cardial fatty acid metab lism in bese rats. Meth ds: SDmale rats were given six weeks t build a high- fat diet besity m del. They were rand mly divided int bese c ntr l( OC) gr up and bese exercise( OE) gr up, and after the latter accepting aer bic endurance exercise f r 12 weeks were measured heart weight index, my cardial free fatty acid ( FFA) c ncentrati n by the way f c pper c l r determinati n, H- FABP c ntent with the meth d f West