秀丽线虫物理低氧损伤模型的建立与应用_任长虹.pdfVIP

,2011,27(3) 257 * 1+ 1,2+ 1 1 2 2v 1,2v 任长虹 , 张继业 , 石锦平, 蒋 斌, 赵 娜 , 刘虎岐 , 张成岗 ( 1. , , , 100850; 2. , 712100) = : 利用秀丽线虫研发合适的低氧 伤模型, 以更好地揭示低氧生理和低氧病理的分子机制: 通过对秀丽线虫进行不同时间的低氧处理,系统观察线虫的死亡率运动功能细胞形态及相关蛋白表达水平的变 化,分析低氧对线虫的 伤情况: 氧浓度为0.2%的物理性低氧可引起秀丽线虫多种细胞形态发生变化, 进 而导致线虫死亡,且死亡率随低氧时间延长而持续增加, 同时低氧诱导因子(HIF-1) 的表达显著上调进 一步通过 神经元特异性转基因(绿色荧光蛋白) 株系观察到低氧可引起神经元特征性 伤: 成功建立有效简便易行 的线虫物理低氧 伤模型,可用于低氧病理和低氧应答分子机制研究 = 秀丽线虫; 物理低氧; 转基因线虫; 物理低氧模型 = R285. 5 = A = 1000-6834( 2011)03-257-06 Development and application of the physical hypoxic models of C. elegans 1 1,2 1 1 2 REN Chang-hong , ZHANG J-i ye , SHI Jin-ping , JIANG bin , ZHAO Na , 1v 1, 2v LIU Hu-qi , ZHANG Cheng-gang (1. Beijing Instituteof Radiation Medicine, State Key Laboratory of Proteomics, StateKey Laboratory of Medical Neurobiology, Beijing 100850, China; 2. Department of Biology College of Life Science, Northwest Agriculture and Forestry University, Shaanxi712100, China) = ABSTRACT Objective: Todevelop asuitable hypoxicinjury model, which is important for revealing pathological molecular mechanism of hypoxia. Methods: Wefocused onC. elegans bytreatment with different hypoxictimes and systematically observedmortality, movement, cel- lular morphology and the related-protein expression of the animals. Res lts: We demonstratedthat hypoxia (0.2% partial pressureof oxygen) induced morphological cell defects, and then leading to death of C. elegans. The mortality of C.