肿瘤学研究常用方法.pptVIP

肿瘤学研究常用方法 What Kind of Disease Is Cancer? Control of G1 phase Control of S phase Control of G2phase Control of M phase Control of G1 phase Control of S phase Checkpoint (DNA synthesis on、off ) Cyclin－CDK PCNA Cyclin－CDK PCNA  Control of M phase  Checkpoint MPF的活性调节 oncogene的过表达 antioncogene 的失活 cyclin基因和表达蛋白的异常 cdki基因和表达蛋白的异常 Cell Proliferation Kinetics 检测方法 1. Cell Counting 四唑盐法 2. Growth Curve 3. Doubling Time Computed Tomography 某转移癌： Do ＝2，Dt＝4，t＝90 DT = 0.1 × 90 / 0.6 - 0.3 = 30(d) effective doubling time, TD eff：考 虑细胞丢失因素的