ELISA法检测HBsAg出现假阳性的原因探讨.pdfVIP

2010年7月第48卷第19期 ·检验与临床· ELISA法检测I-IBsAg出现假阳性的原因探讨 吴玉红 (北京大学深圳医院检验科，广东深圳518036) (4，52)，x毛8．34，PO．05。结论ELISA法检测乙肝HBsAg灵敏度高、特异性强，易造成假阳性。 f关键词1ELISA法；HBsAg；假阳性；原因 【中图分类号】IL512．62 【文献标识码】A 【文章编号】1673—9701(2010)19—81--02 Immunosorbentof BSurface Enzyme-linked AssayHepatitis OccurredBecauseofFalsePositive WU Yuhong Shenzhen I．abomtary，PekingUniversity Hospital，Shenzhen518036，China To thedetectionof ELISA，falsereason．MethodsELISA ofthedifferent [AbstractlObjectiveinvestigate HBsAgby positive assay the1ale0f Bsuff址e withthetreeof agegroups an衄enpositive，and for hepatitis colloidal洲standardassay EUSAdetectionof rate of thanthestandardmethod hepatitisB鲫rb饱antigenpositivesensitivity higher 92．3％(48／52)．significantly of B 0f colloidaldetection 8mke WAg using gold hepatitis positiverate，sensitivity 96．0％(430／448)。 anon 72．7％(48／66)，specificity lowerthanthe standardmethod the of EUSA of B false slightly gold using specificitydetection．Byassayhepatitissut白∞antigen rate thefalse detectionof w∞4．02％(181448)and ELISA positive negative B and leadtofalse hepatitis high specificity，could HBsAgsensitivity easily positive． 【Keywords】EUSAmethod；HBsAg；False-positive；Reasons