恩施硒矿区土壤亚硒酸盐还原菌地多样性研究.pdfVIP

恩施硒矿区土壤亚硒酸盐还原菌的多样性研究 王 丹 粟静 宋玉梅 张磊磊王 革 娇 郑世学 华中农业大学农业微生物学国家重点实验室，生命科学技术学院，武汉，430070 摘要：硒(Se)是生命体所必须的非金属微量元素。自然界中的硒主要以4种 价态存在：还原态的硒化物 （-2）、单质硒(0)和氧化态的亚硒酸盐(+4)及硒 酸盐(+6)。其中，亚硒酸盐易溶于水，毒性最强。亚硒酸盐还原菌可以将亚硒 酸盐还原为红色单质硒，既可用于某些硒环境污染的生物治理，其产物单质硒在 生物补硒方面也有应用价值。另外，单质纳米硒有很活跃的光学电子特性，是未 来 “纳米技术”中的良好研究材料。 Isolationandcharacterizationofanendosulfan-degrading strain,Stenotrophomonassp.LD-6,anditspotentialinsoil bioremediation FangboYu1 ShinawarwaseemaLi2JingyaSun3 LinpingLuo1 1DepartmentofEnvironmentalSciences,CollegeofEnvironmentandResourceSciences, ZhejiangAgriculturalandForestryUniversity,Linan,China 2InstituteofAgriculturalSciences,UniveristyofthePunjab,Punjab,Pakistan 3DepartmentofEnvironmentalSciences,SchoolofMarineSciences,ZhejiangOcean University,Zhoushan,China Abstract:Aerobicbacteriadegradingendosulfanwereisolatedfromcontaminated sludge.Oneoftheisolates,LD-6,wasidentifiedasStenotrophomonassp.The bacteriumcouldutilizeendosulfanasthesolesourceofcarbonandsulfur.100mg/l endosulfanwascompletelydegradedwithin10days,andendosulfandioland endosulfanetherweredetectedasmajormetaboliteswithaslightdecreaseinculturepH. TheresultsindicatedthatS.sp.LD-6mightdegradeendosulfanbyanon-oxidative pathway.Biodegradationofbothisomerswasrelativelybetteratatemperaturerangeof 25-35℃,withamaximumat30℃.Inaddition,cellcrudeextractofstrainLD-6could metabolizeendosulfanrapidly,anddegradativeenzymeswereintracellulardistributed andconstitutivelyexpressed.Besides,applicationofthestrainwasfoundtopromotethe removalofendosulfaninsoil.Thisstudymighthelpwiththefutureresearchinbetter understandingofthebiodegradation. Keywords:Stenotrophomonassp.;endosulfan;biodegradation