脊髓损伤对大鼠骨细胞凋亡的影响_医学论文.docVIP

脊髓损伤对大鼠骨细胞凋亡的影响_医学论文 脊髓损伤对大鼠骨细胞凋亡的影响_医学论文 作者：李靖，王全平，王哲，范宏斌，陶惠人 【关键词】 脊髓损伤 　　关键词: 脊髓损伤骨质疏松凋亡骨细胞 　　摘 要:目的 探讨脊髓损伤（SCI）对骨细胞凋亡的影响及其在SCI后骨质疏松发病中的可能机制. 方法 20只3mo龄SD大鼠均分为SCI组与对照组.SCI组于T10 椎体处完全横断脊髓对照组仅行椎板切除术，3wk后对股骨进行取材.应用原位凋亡法计数股骨髁部松质骨骨细胞的凋亡指数对股骨髁部行透射电镜观察采用双能X线骨密度仪对各组大鼠的股骨髁部骨密度（BMD）进行测定. 结果 SCI组BMD较对照组明显降低（0.05），小梁骨中骨细胞凋亡比率显著上升（0.001），透射电镜观察到SCI组骨细胞异染色质凝聚、边集等典型的细胞凋亡征象. 结论 骨细胞凋亡在SCI后骨质疏松发病中起着重要作用. 　　Keywords:spinal cord injuryosteoporosisapoptosisos-teocyte 　　Abstract:AIM To investigate osteocyte apoptosis in the trabecular bone of rats with spinal cord injury（SCI）and its role in the pathogenesis of osteoporosis following SCI.METHODS Twenty12-week-old rats were randomly divid-ed into the SCI group and control group with10in each group.Rats in the experimental group underwent spinal cord transection at the tenth thoracic vertebrae.Rats in the con-trol group underwent a sham procedure.All animals were sacrificed at3weeks postoperatively.Dual-energy X-ray ab-sorptiometry was carried out to determine the bone mineral density（BMD）in distal femur and ultrastructural investiga-tion of trabecular bone in distal femur was performed by means of transmission electron microscopy.The apoptotic os-teocytes were detected by using terminal-deoxynucleotidyl mediated nick end labeling（TUNEL）technique.RESULTS BMD was lower in SCI group than that in control group（0.05）apoptotic index of osteocytes was significantly high-er in the SCI group than in the control group in trabecular bone（6.84%vs1.98%，0.001）.Condensation and margination of heterochromatins，the typical features of apoptosis，were obviously seen in osteocytes in SCI group.CONCLUSION Rise in apoptotic rate of osteocytes follow-ing SCI indicates that apoptosis may play a part in the patho-genesis of osteoporosis after SCI. 　　0 引言 　　细胞凋亡是指有别于细胞坏死，由基因调控的细胞有序的死亡方式.机体大部分细胞是以此种方式完成新旧细胞更替和组织更新.构成组织的细胞异常凋亡可导致肿瘤、退行性变等许多疾病的发生.近年研究表明雌激素缺乏所致的骨质疏松的发病与骨细胞的异常凋亡有关［1，2］ .我们通过动物实验探讨骨细胞凋亡在脊髓损伤（spinal cord injury，SCI）后骨质疏松发病过程中的可能作用.