小麦孢囊线虫病生防真菌08F04菌株的鉴定及防效测定.pdfVIP

29(4)509-514 中国生物防治学报 Chinese Journal of Biological Control 2013 年 11 月 小麦孢囊线虫病生防真菌08F04菌株的鉴定及防效测定 张 洁，袁虹霞，孙炳剑，陈 莉，付 博，李洪连* （河南农业大学植物保护学院，郑州 450002 ） 摘要: 小麦禾谷孢囊线虫病已成为我国黄淮麦区的重要病害，目前生产上尚缺乏有效的防控方法。菌株 08F04 是作者从禾谷孢囊线虫孢囊上分离的 1 株寄生真菌。本研究通过形态学和分子生物学方法，将其鉴 定为球孢白僵菌 Beauveria bassiana (Bals.) Vuill 。2011 和 2012 年对该菌株进行了盆栽和田间防效试验，菌 株 08F04 麦粒砂培养物 6% （w/w ）土壤处理的盆栽试验孢囊减退率分别达到65.03%和 50.95%；田间试验 中，菌剂08F04 20 g·m−2 沟施处理土壤，2011 年在许昌和荥阳的孢囊减退率分别为 58.28%和 32.13% ，2012 年分别达到 58.49%和 44.50% 。结果表明，菌株 08F04 是防治小麦禾谷孢囊线虫病具有较大潜在开发价值 的生防菌株。 关 键 词：小麦禾谷孢囊线虫病；生物真菌；球孢白僵菌；孢囊减退率 中图分类号：S476.12 文献标识码：A 文章编号：1005-9261 (2013)04-0509-06 Identification and Effects of Biocontrol Fungal Strain 08F04 on Cereal Cyst Nematode of Wheat * ZHANG Jie, YUAN Hongxia, SUN Bingjia, CHEN Li, FU Bo, LI Honglian (College of Plant Protection, Henan Agricultural University, Zhengzhou 450002, China) Abstract: Cereal cyst nematode (CCN) has become a serious disease in Huanghuai wheat production area. The biocontrol strain 08F04 is parasitic fungus which was isolated from the cyst in the diseased fields. It was identified as Beauveria bassiana (Bals.) Vuill by morphology and rDNA-ITS sequence analyses. Its control efficiency on CCN was evaluated in the greenhouse and diseased fields (Xuchang and Xingyang), respectively. The cysts decreasing rates were 65.03% and 50.95% respectively in 2011 and 2012, when the strain 08F04 mixed with soil (6% w/w) in pots. In field trial, the cysts decreasing rates in 2011 were 58.28% and 32.13% in Xingyang and Xuchang respectively, and in 2012 were 58.49% and 44.50% respectively. The fungal strain 08F