生物安全柜的选择，安装与使用.pdf

Appendix A – Primary Containment for Biohazards: Selection, Installation and Use of Biological Safety Cabinets Section I—Introduction This document presents information on the design, selection, function and use of Biological Safety Cabinets (BSCs), which are the primary means of containment developed for working safely with infectious microorganisms. Brief descriptions of the facility and engineering concepts for the conduct of microbiological research are also provided. BSCs are only one part of an overall biosafety program, which requires consistent use of good microbiological practices, use of primary containment equipment and proper containment facility design. Detailed descriptions of acceptable work practices, procedures and facilities, known as Biosafety Levels 1 through 4, are presented in the CDC/NIH publication Biosafety in Microbiological and Biomedical Laboratories (BMBL).1 BSCs are designed to provide personnel, environmental and product protection when appropriate practices and procedures are followed. Three kinds of biological safety cabinets, designated as Class I, II and III, have been developed to meet varying research and clinical needs. Most BSCs use high efficiency particulate air (HEPA) filters in the exhaust and supply systems. The exception is a Class I BSC, which does not have HEPA filtered supply air. These filters and their use in BSCs are briefly described in Section II. Section III presents a general description of the special features of BSCs that provide varying degrees of personnel, environmental, and product protection. Laboratory hazards and risk assessment are discussed in Section IV. Section V presents work practices, procedures and practical tips to maximize information regarding the protection afforded by the most commonly used BSCs. Facility and engineering requirements needed for the operation of each type of BSC are presented in Section VI. Section VII reviews requirements for r