铀矿石冶金菌优势菌株的研究.docVIP

毕业设计(论文) 中文题目：铀矿石冶金菌优势菌株的研究 学生姓名：郭 勤 学 号： 060552203 指导教师：李 江 专 业： 生物技术 二零一零年 六 月 Graduation project(paperS) English title：Uranium mine metallurgy fungus superiority strain research Name : Guo qin Number : Instructor : Li jiang Major : Biotechnology June，2010摘 要 嗜酸性微生物是一类生活在极端酸性环境下的微生物，，，SJH为底层添加菌，，6种嗜酸性氧化亚铁硫杆菌（暂定名为：A1，A2，A3，A4，A5，A6），1株氧化硫硫杆菌（暂定名为：B1），3株异养菌（暂定名为：C1，C2，C3）根据各菌株在不同平板表面的菌落形态特征，16SrRNA基因扩增技术对所获得的菌种进行了初步鉴定。群落中占优势的嗜酸性微生物为氧化亚铁硫杆菌(Acidithiobacillus ferrooxidans，A. f) 。对其中一株氧化亚铁硫杆菌（A6）的培养温度、接种培养基初始pH值及接种量对其生长状况的影响做了单因素实验，结果表明，A6氧化Fe2+最快的培养温度为35℃、最适初始pH值为2.3。最佳接种量为10% 。初步研究了底物S、Na2S2O3和Na2SO3对氧化硫硫杆菌B1的生长的影响，结果表明最适合B1的底物为Na2S2O3，其次为单质S。 关键词： 嗜酸性微生物；；氧化亚铁铁杆菌；氧化亚铁硫杆菌 Abstract The acidophilia microorganism is a kind of life under the violent acidic environment microorganism, has widely used in the biological metallurgy. Has carried on the separation and the preliminary research and the discussion to the Jiangxi some uranium mine acidophilia microflora superiority fungus. this article to picks piles from Jiangxi some ore soaks experiments six samples: S1, S2, S3, S4, S5, S6 carries on the analysis in the 9K+S+Fe liquid media to its mix bacteria colony ferric oxides efficiency, finally discovers S3, the ferric oxide efficiency is best. Utilizes the double-decked solid plating technique, take the agarose as the peptizer, take SJH as the first floor increase fungus, separates in the S3 sample the microorganism, obtains 6 kind of acidophilia ferrous oxide thiobacilli (A1, A2, A3, A4, A5, A6), 1 sulphur oxide thiobacillus (B1), 3 heterotrophic bacterias (C1, C2, C3). According to the different fungus in the different dull surfaces colonial morphology characteristic, the microstructure, and unifies the 16SrRNA gene to increase the technical appraisal mold mushroom spawn. Gets the advantage the acidophilia microorganism for the ferrous oxide thiobacillus (Acidithiobacillus ferrooxidans, is called A. f). To ferrous oxide thioba