实验性肝硬化大鼠细胞因子IL18、TNFα、IFNγ的变化及意义_临床医学论文.docVIP

实验性肝硬化大鼠细胞因子IL、TNF、IFN的变化及意义_临床医学论文 实验性肝硬化大鼠细胞因子IL、TNF、IFN的变化及意义_临床医学论文 作者：党双锁，高宁，程延安，边静，王顺达，孙明珠 【摘要】 　　目的 探讨在四氯化碳(CCl4) 复合因素诱导实验性肝硬化大鼠的形成过程中，白介素18(IL、肿瘤坏死因子α(TNF、γ干扰素(IFN的变化及意义。方法 将80只清洁级雄性SD大鼠随机分为正常对照组和造模2周组、4周组、6周组，每组20只。予以CCl4复合因素诱导大鼠肝硬化，并在2、4、6周3个时间点分别处死6只大鼠。采用ELISA法检测大鼠血清IL、TNF、IFN和肝脏组织匀浆上清液中的IL含量；HE染色观察肝脏的组织病理学变化。结果 ①CCl4复合因素诱导的实验性大鼠，组织病理学观察发现，2周时大鼠肝脏组织细胞肿胀变性，6周时有大量纤维增生，部分肝组织有假小叶的形成；②随着造模时间的延长，实验大鼠血清IL、TNF、IFN水平逐渐升高，造模6周组与正常对照组比较差异有统计学意义(0.01)；③造模组肝脏组织匀浆中IL随肝损害程度的加重而升高，造模6周组与正常对照组比较差异有统计学意义(0.01)。结论 在CCl4复合因素诱导大鼠肝硬化的形成过程中，IL、TNF、IFN水平逐渐升高，提示该3种细胞因子与肝硬化的形成及发展有关。 【关键词】 大鼠；肝硬化；白介素18；肿瘤坏死因子α；γ干扰素 　　ABSTRACT: Objective To investigate the changes of interleukinhosis induced by the composite factors of carbon tetrachloride (CCl4) in SD rats and their significance. Methods Totally 80 male SD rats of clean class were randomly divided into normal control group (20 rats) and model groups, the latter of which were further divided into three groups according to the length of administration time, namely, 2week group (2 wk group), 4week group (4 wk group) and 6week group (6 wk group), with 20 rats in each. Six rats were killed after 2 wk, 4 wk and 6 wk administration time, respectively. The rat serum levels of IL18, TNFα and IFNγ and the hepatic homogenate supernatant of IL18 were detected by ELISA; pathological changes of liver tissues were observed by HE staining. Results ① Pathological observation revealed that in the model groups hepatic cells degenerated and swelled at week 2 while large amounts of fibrosis and pseudolobules of some liver tissues occurred at week 6. ② The serum levels of IL18, TNFα and IFNγ were gradually increased with the modeling time, and they were significantly higher in 6week group than in normal control group (0.01). ③ The levels of hepatic homogenate supernatant of IL18 in the model groups were elevated with liver damage, and they were significantly higher in 6week group than in normal control group (0.01). Conclusion During the f