优化的ATP生物发光法快速检测面粉中细菌总数.pdfVIP

ofAnhui 安徽农业科学，Journal I。39(7)：4055—4057 责任编辑郑丹丹责任校对马君叶 A一．Sci．201 优化的ATP生物发光法快速检测面粉中细菌总数 李利霞，伍金娥。，常超，张佳艳，王凌 (武汉工业学院食品科学与工程学院．湖北武汉伽23) 摘要[目的]建立一种快速检测面粉中细茵总数的方法。[方法】采用优化的A1限生物发光法检测面粉中的细菌总教，考察D一荧先 素(L皿)浓度、萤火虫荧光素酶(FL)浓度、Mf+浓度对生物发光反应的影响。[结果]I』l浓度为70n∥L，FL浓度为50mg／L．Mg+浓度 为0．2500m“mL／L时，ATP生物发光法的测定结果较理想。该法最低检测细菌总数为1．0×103clu／r，II，与传统的平扳计数法共有良好 茵总数。 关键词AW生物发先法；平板计数法；面粉；细菌总数 中图分类号$37文献标识码A 文章编号晒17—66ll(加11)07—04055—03 Research011the DetectionofBacterialCount蛔Flourwim A什BioluminesemtMethod Rapid Optimal uLi-xiaetal ofFoodScienceand Engineering，WuhanPolytechnicUIIiversi‘y，Wuhan，Hubei (CoUege 430023) Abstract detectionmethodofthebacterialcountinflourwas Thebacterialinflourwas [Objective]Therapid established．[Method1 Count testedwith A11Pbioluminescentmethodandtheinfluencet11e of and ATP optimal of concentrationLn．FLMg“on bioluminesceneeresponse was results that Ln indicatedwhentheconcentrationof was70 and 0 studied．[Result】11le me／L；FL，50mg／L Mg“，0．2．50mmoVL，the result the X determinationfrommetllodWas minimumofbacterialcount(1．0105cfu／m1)couldbedetectedwiththe relativelyperfeCt．珏e wascorrelatedwellwiththemethodofconventional was Thismethod method，which platecount(P0．05)and冗sD4．0％。【Conclusion1 懈withcharacteristicsoflow and forthe detectionofbacterial CO_$t，easyoperation。fastresponse。accuratereliable。which惴capablerapid countinfood． words method：Platecount A口bioluminescent count Key method；