毕业设计（论文）-高效液相色谱法同时测定食品中八种添加剂的研究.doc

 本科生毕业论文（设计） 题 目: 高效液相色谱法同时测定 食品中八种添加剂的研究 目 录 摘要……………………………………………………………………………………………1 关键词…………………………………………………………………………………………1 Abstract………………………………………………………………………………………2 Key words………………………………………………………………………………………2 一、前言……………………………………………………………………………3 二、材料与方法……………………………………………………………………………7 （一）试剂…………………………………………………………………………7 （二）设备与仪器……………………………………………………………………………7 （三）色谱条件的选择………………………………………………………………………7 （四）标准溶液配制…………………………………………………………………………10 三、结果与讨论……………………………………………………………………………12 （一）各组分定性……………………………………………………………………………12 （二）标准曲线及检出限……………………………………………………………………14 （三）回收率试验……………………………………………………………………………16 四、结论………………………………………………………………………………………18 致谢…………………………………………………………………………………………19 参考文献……………………………………………………………………………………20 高效液相色谱法同时测定食品中八种添加剂的研究 摘要：采用高效液相色谱法同时测定食品中安赛蜜、苯甲酸、山梨酸、糖精钠、对羟基苯甲酸甲酯、对羟基苯甲酸乙酯、对羟基苯甲酸丙酯、对羟基苯甲酸丁酯。应用Zorbax SB-C18（4.6×150mm i.d，5μm）柱,以甲醇-0.02M乙酸铵溶液为流动相进行梯度洗脱，流速为1.00mL/min;柱温:25℃;紫外检测波长0-9min为230nm，9min后为254nm。8种组分的回收率在97.8%～102.4%之间,RSD均小于3%。该方法操作方面，结果准确，重现性好，能够满足食品中8种组分的同时测定。 关键词：高效液相色谱法; 安赛蜜; 苯甲酸; 山梨酸; 糖精钠; 对羟基苯甲酸酯类 Study on the simultaneous determination of eight food additives in food by high performance liquid chromatographic Student majoring inFood Science and Engineering zhang xia Tutor tian qi ying Abstract：A high performance liquid chromatographic (HPLC) method was developed for the determinationofSacchar,Benzoic-acid,Sorbicacid,Sodium-cyclamate,Methyl-p-hydroxybenzoate,ethyl-p-hydroxybenzoate,propyl-p-hydroxybenzoate,butyl-p-hydroxybenzoate.The chromatographic analysis was carried out using a Zorbax SB-C18 (4.6×150mm i.d, 5μm) column. The mobile phase was ammonium acetate aqueous solution-methanol by gradient elution at 1.00 mL/min. The eluation was monitored by UV detector at 230 nm at first 9 mins and 254nm later. The recoveries were 98.3%～102.4% and the relative standard deviations (RSD) were gwnerally below 3.0%.. The result showed that it was a rapid and accurate method for the determination o