丹酚酸B与高氧液对兔肢体缺血再灌注氧化应激损伤的影响.docVIP

丹酚酸B和高氧液对兔肢体缺血再灌注氧化应激损伤的影响 涂泽松陈志维李逸群 广东省佛山市中医院骨科 528000 [摘要】目的探讨高氧液和丹酚酸B对肢体缺血再灌注损伤的影响。方法选用健康新西兰家兔24只，随机分 为4组，在缺血前从耳缘静脉推注等量的生理盐水 A组 、高氧液 B组 、丹酚酸B C组 或高氧液加丹酚酸B D组 ，夹 队股动、静脉，建立肢体缺血再灌注损伤模型，在缺血前和再灌注4h抽血检浏MDA, SOD，取排肠肌作病理检浏。结果 血清丙二醛浓度较前明显升高，高氧液和丹酚酸B可以抑制其升高 P 0.01 ，两者有协同作用 尸 0.01 ;血清超氧 化物歧化酶活性较前明显降低，高氧液和丹酚酸B可以抑制其降低 P 0.01 ，两者有协同作用 尸 0.01 。骨骼肌 HE染色见:相对于对照组其他组骨骼肌损伤程度较轻，以联合用药组最轻。结论高氧液和丹酚酸B能抑制肢体缺血 再灌注氧化应激损伤，而且两者有协同作用。 【关键词】高氧液;丹酚酸B;缺血再灌注损伤 Effects of salvianoli acid B and hyperoxic solution on experimental ischemia reperfusion坷ury TU Ze一song, CHEN Zhi一wei, !l Yi一，un . Department of Orthopedics，Foshan Traditional Chinese Medicine Hospital，Foshan 528000, China 【 Abstract Objective Aim。investigate the elfeds of salvianoli acid B and hyperoxw solution on mpeamerual ischemia reperfusion iruury of extremity. Method Tweet，一four healthy New Zealand rabbits~divided into four groups randomly. Equivalent 0. 9%ONaCI group A ，hyperoxic solution group B , salvianoli acid B group C ，Salvianoli acid B and hyperoxic solution~injected through， 吨e vein before ischemia. The femoral artery and vein~blocked and set tip the model of ischemia reperfusion injury of extremity. Before ischernia and after 4 hours’mperfusion, the blood samples~taken to measure the levels of blood malondialdehyde MDA supermide dismutuse SOD .The gastrocnemius muscles~obseruetl by pathology test. Results Serum eruymatic activity of malondzaMehyde MDA ~~obviously. Salvianoli acid B and hyperoxic solution can precwu the~了试P 0.01 . They present~ P 0. 0l . Serum Enzymatic activity of superoxide dismutase SOD reduce obviously. Salvianoli acid B and hyperoxic solution can pre- vent the reduction of it P 0. 01 . They present interaction P 0.01 .The test of HE staining show that other groups，lighter than the control group at degree of skeletal muscle uyury. The groupD~the lightest of them. Conclusions Salvwrwli acid B and hyperoxw solu- tion~prevent the lipid peroxidation in ischernia reperfusion uyruy. They pr