西府海棠花粉生活力测定.pdfVIP

, J urnal fAnhuiAgr.i Sci. 2011, 39( 6): 3423- 3425 李菲菲 况玲玲 尤扬, 贾文庆 (, 453003) [目的]研究分析了不同染色方法和离体萌发法对西府海棠花粉生 力的影响, 以期为选出影响西府海棠花粉生 力的最佳方法 提供理论依据[方法]以西府海棠花粉为试材, 利用碘 -碘化钾染色法醋酸洋红染色法离体萌发法和 TTC(氯化三苯基四氮唑)染色 法对花粉生 力进行测定, 并研究了不同浓度的钙对花粉萌发和花粉管生长的影响[结果]适合西府海棠花粉萌发和花粉管生长的最 佳培养基是: 0. 8 g琼脂+ 15. 0 mg/L蔗糖+ 2. 0 mg/L硼酸; 低浓度的钙对花粉萌发和花粉管的生长有促进作用, 高浓度的钙对花粉萌 - 5 发和花粉管的生长有抑制作用, 培养基中Ca(NO ) 浓度为 1. 6 10 mg/L时最适合西府海棠花粉萌发和花粉管的生长[结论] 3种 3 2 染色方法均不适合测定西府海棠花粉生 力 西府海棠; 花粉; 生 力; 测定方法 S685. 99 A 0517- 6611( 2011) 06- 03423- 03 M easurement on Pollen Vitality ofMa lus m icroma lus Makino YOU Yang et al ( C llege fL andscape A rchitecture, H enan Institute f Science and Techn l gy, X inxiang, H enan 453003 ) Abstract [ Objective] The study ami ed t study the effects f different dyeingmeth ds and the vitr p llen germ inati n meth ds n the p llen vitality fM a lus m icrom a lus M ak in s as t pr vide the the ry basis f r ch sing the bestway f affecting the p llen vitality fM. m icroma lus. [M eth d] W ith the p llens fM. m icrom a lus as the testedm ater ials, the p llen v itality fM. m icrom a lus was determ ined by them eth ds f IK I 2 dyeing, canaine acetate dyeing, germ inati n in vitr and TTC (T ripheny l tetraz lium chl r ide) dyeing and the effects f the calcium w ith different c ncn. n the p llen germ inati n and p llen tube gr wth were studied. [ R esult] The bestm edium thatw as advantage us t the p llen germ inati n and p llen tube grw th was as f ll ws: 0. 8 g agar + 15. 0 mg/L sucr se + 2. 0mg/L b r ic acid. The l w c ncn. f calcium had the aux acti n n - 5 the p llen germ inati n and p llen tube gr w th and the hig