受PVY 诱导的烟草NtERD1 的基因分离与表达分析.pdfVIP

62 中国烟草科学 Chinese Tobacco Science 2010-10 ，31 （5 ） PVY NtERD1 * 266101 cDNA PVY 745 bp EST Blast ERDORfinder 492 bp 163 Blastp NtERD1 GenBank GU144573PCR NtERD1 PVY NtERD1 PVY PCR PVY TS413 1007-5119201005-0062-06 DOI 10.3969/j.issn.1007-5119.2010.05.013 Gene Isolation and Expression Analysis of NtERD1 Induced by PVY in Tobacco (Nicotiana tabacum L.) * CHEN Shuai, LIU Guanshan , YANG Aiguo, WANG Yuanying, SUN Yuhe (Key Laboratory of Tobacco Quality Control, Ministry of Agriculture, Tobacco Research Institute, Chinese Academy of Agricultural Sciences, Qingdao 266101, China) Abstract: A 745 bp EST was obtained from the tobacco (Nicotiana tabacum L.) suppression subtractive hybridization (SSH) library induced by PVY (potato virus Y), using SSH and cDNA chip technologies. Sequence alignments with Blast indicated that the EST revealed a high degree of similarity with other members of the plant early responsive to dehydration (ERD) genes in both nucleic and amino acid levels. The cDNA contained an open reading frame (ORF) of 492 bp consisting of 163 amino acids. Amino acid Blastp indicated that the predicted protein had a high degree of homology with other ERD proteins. Thus the new gene screened belongs to tobacco ERD genes and was named as NtERD1 (GenBank accession no.GU144573). The results of real-time PCR showed that NtERD1 was up-regulated in leaves infected with PVY in early stage. Therefore NtERD1 was induced by PVY infection and might play important roles in tobacco resistance to viruses. Keywords: tobacco; ERD gene; real-time PCR; PVY Nicotiana tabacum L. ERD 1 h Y PVY [3]16 ER