涤痰汤对帕金森病大鼠神经细胞凋亡及相关基因表达的影响_丁宏娟.pdfVIP

# 740# 辽宁中医杂志 2010年第 37 卷第 4期 # 实验研究# , (上海中医药大学, 上海 201203) : 目的: 探讨涤痰汤对帕金森病大鼠多巴胺神经细胞凋亡及相关基因表达的影 方法: 应用 6- 羟基多巴 胺立体定向注射制作 PD大鼠模型将成功的 PD模型随机分为模型组与化痰组, 另外纳入正常对照组和假手术组, 分 别给予相应处理, 共 14 天用 TUNEL 测定各组大鼠的细胞凋亡数, 免疫组化染色法测定 Bcl- 2基因和 Bax 基因的表 达结果: 与模型组比较, 化痰组细胞凋亡数明显减少, 差异有显著意义(P 0105); 同时 Bcl- 2 的表达明显增多, Bax 的表达明显降低, 差异均有显著意义(P 010 1) 结论 涤痰汤通过增强 Bcl- 2和降低 Bax的表达, 减少 PD大鼠的神经 细胞凋亡而发挥保护神经元的作用 : 帕金森病; 涤痰汤; 细胞凋亡; Bcl- 2; Bax : R28515 : A : 1000- 1719( 20 10) 04- 0740- 03 Effects ofD itan Decoction on theApoptosis and related Genetic Expression ofRatesw ith Parkinson Disease D ING H ong-juan, H E J ian-cheng (ShangH ai Univ ersity of Traditiona Ch inese M ed ic ine, Shanghai 201203, Ch ina) Abstract: O bj ectiv e: T study the effects f Ditan dec cti n n the ap pt s is and related genetic expressi n f rates w ith Par- k ins n d isease.M ethods: The PD m del rats were established thr ugh stere t x icm icr injecti n f 6- OHDA. The successfulm d- els f PD w ere rand m ized int the m del gr up and the elmi inated ph legm gr up, and w e br ught int the n rm al c ntr l gr up and the sham- perated gr up. Every gr up was given relevant treatm ent f r 14 days. Then we c un ted the number f ap pt sis f each gr up w ith TUNEL and determ ined the expressi n f Bcl- 2 and Bax gene w ith mi mun hist chem istry. R esu ts: C m pared w ith the m del gr up, the number f ap pt s is f the elmi inated phlegm gr up is sign ificantly reduced, the difference is sign if-i cance(P 01 05), m eanwh ile the expressi n f Bcl- 2 gene s ignificantly increased and Bax gene sign ifican tly reduced, the d iffer- ence is sign ificance (P 010 1). Conc usion: D itan dec cti n c uld mi pr ve the expre