呼吸重症监护病房多重耐药铜绿假单胞菌耐药基因研究.pdfVIP

·143l· ．论著． 呼吸重症监护病房多重耐药铜绿假单胞菌 耐药基因研究 阎锡新 赵媛媛 祁春艳 潘文森 张鲁涛 【摘要】 目的 观察我院呼吸重症监护病房(RICU)临床分离多重耐药铜绿假单胞菌B一内酰胺酶基 因和I类整合子标志物(qaeEAl-sull)存在状况。采用多基因聚类分析方法进行多重耐药菌株亲缘性 分析。方法收集RICU分离多重耐药铜绿假单胞菌33株．采用PCR方法检测8种口一内酰胺酶基因 分析法分析菌株亲缘性。结果 33株多重耐药铜绿假单胞菌中bIaTEM阳性8株(24．24％)，blaVIM 聚类分析发现存在克隆传播现象。结论我院RICU多重耐药铜绿假单胞菌携带多种B一内酰胺酶基因。 以携带blaTEM基因为主。qacEA卜sull携带率高．聚类分析湿示存在克隆传播现象。应引起临床重视。 【关键词】多重耐药；铜绿假单胞菌；耐药基因；聚类分析 of resistantPseudomonasinRICUYANXi—xin。， Drug-resistant aeruginosa genesanalysismulti-drug zHAO Wen—sen．zHANGLH—tao．‘∞leInstitute Yuan—yuan．QlChun—yah，PAN ofRespiratory DiseasesHebei Medicine．theSecond Hebei of Province．DepartmentofRespiratory Hospitalof Medical 050000。olina University．Shijiazhuang To the rateofbeta—lactamaseand [Abstract]Objectiveinvestigatepositive qac△卜sullgenesby chainreaction(PCR)methodclinicalisolatedstrainsof resistant polymerase among multi—drug Pseudomonas were cluster aeruginosa．Thestrains’consanguinityinvestigatedbymulti—genesanalysis． of were from Methods strains resistantPseudomonas isolated Thirty-three multi·drug aeruginosa in TEM。SHV，PER。DHA．IMP。VIM。OXA一23· hospitalizedpatientsRICU．p—Lactamasegenesincluding OXA一24and detectedPCR were by qacEA卜sull，wereby amplication．Thestrains’homologyinvestigated cluster PCRresultsshowedthe ratesOf blaTEM．blalMP， multi—genesanalysis．Results positive genes