犬心房肌细胞分离的方法学探讨.pdfVIP

# 436# 中国心脏起搏与心电生理杂志 20 0年第 24卷第 5期 * 张玲 宋伟 马艳红 段明军 侯月梅 [] 12, Langendorff, , 10 s, , , , , 5 min, 125U /mlÒ 200 m l 30~ 45 m in, 5 min, , KB,, 5m in, 200, , 1 h, 90%, [] ; ; ; ; O I: 10. 3969/ .j issn. 1007- 2659. 2010. 05. 018 + R331. 3 8 A 1007- 2659( 2010) 05- 0436- 03 Isolation of atrial myocytes from canine. ZHANG L ing, SONG W ei,MA Yan-hong, UAN M ing-jun, HOU Yue-mei. e- partment ofCardiology, the F irstA ffiliatedHospital ofX injiangM edicalUniversity, U rumqi830054, China Corresponding author: HOU Yue-me,i E-mai:l houyueme@i sina. com [ Abstract] Objective To establish a practical and reliable technique of atrialmyocytes isolation from canine for ion currents research. M ethod Twelve hearts from healthy canine were intubated through left circumflex branch and per- 2+ fused for 10 s by the Langendorff perfusion apparatusw ith normalTyrodecs solution, thenw ith Ca -free Tyrodecs solution for 5m in andw ith enzyme solution containing typeÒ collagenase 125U /ml200ml for 30~ 45m in, atrium werewashed by 2+ Ca -free Tyrodecs solution for 5m in, afterwhich the atrium werem inced into smallpieces in KB solution, dispersed and incubated for 5 m in, and then filtered with 200 screenmesh. The isolated myocyteswere recovered to normal calcium con- centration gradually, and then stored inKB solution at room temperature for1h before patch clamp exp