Argonaute-2蛋白在膀胱移行上皮癌组织中的表达及其临床意义.pdfVIP

l18 JModUrol。Vo1．20No．2Feb．2015 · 基础研 究 · Argonaute一2蛋白在膀胱移行上皮癌组织中的表达及其临床意义 李 军 。，李 伟 ，杨丰强 ，鄢 阳。，车建平。，刘 敏 ，郑军华 (1．上海市浦东新区人 民医院泌尿外科 ，上海 201200；2．同济大学附属第十人 民医院泌尿外科 ，上海 200072) Expression and significanceofArgonaute。-2protein inbladdertransitionalcellcar_‘ cinoma LIJun～，LIWei，YANG Feng—qiang ，YAN Yang。，CHEJian—ping ，LIU M in。，ZHENG Jun—hua (1．DepartmentofUrology，People’SHospitalofPudongNew Area，Shanghai201200，China；2．Department ofUrology，TenthPeople’SHospital，TongjiUniversity，Shanghai200072，China) ABSTRACT：Objective ToinvestigatetheexpressionofArgonaute一2proteininhumanbladdertransitionalcellcarcinoma (BTCC)andtheclinicalsignificance．Methods ExpressionofArgonaute-2proteinwasassessedbyreal—timequantitative PCRandimmunohistochemistry(IHC)inthetumortissueandadjacentnon—cancertissuefrom88patientswhohadundergone surgeryforhistologicallyprovenBTCC．Thecorrelationwithotherclinico-pathologicfactorswereevaluated。Results The expressionofArgonaute一2mRNA andArgonaute一2proteinwassignificantlyhigherintumortissuesthaninadjacentnon-cancer samples(bothP O．05)．A significantcorrelationexistedbetweenthehighexpressionofArgonaute一2proteinwiththepatho— logicalgrading，clinicalstage(T)andlymphnodemetastasis．SurvivalanalysisbyKaplan—M eiersurvivalcurveandlog—ranktest demonstratedthatelevatedArgonaute-2expression incancertissuepredictedworseoverallsurvival(OS)compared togroup withlowerexpression (86．3 VS．62．2 )．Conclusions TheaberrantexpressionofArgonaute一2inbladdertransitional cellcarcinoma ispossiblyinvolvedwith tumorigenesisanddevelopment，and theArgonaute-2protein couldactasapotential biomarkerforprognosisassessmentofbladdercancer． KEY W ORDS：bladdercancer；Argonaute．2protein；real—timequantitativePeR；immunohistochemistry 摘要 ：目的 研究Argonaute-2蛋 白在膀胱移 上皮癌组织 中的表达及临床意义。方法 通过实时荧光定量 PCR、免疫组化法 测定88例经病理确诊的膀胱移行上皮癌患者配对的肿瘤组织、癌旁组织中Argonaute一2蛋 白的表达，分析该蛋 白表达与预后和 其他临床病理参数 的相关性 。结果 膀胱移行上皮癌组织 中Argonaute一2mRNA表达 明显高于癌旁组织 (P