L_谷氨酰胺高产菌的选育和发酵条件.pdfVIP

9 1 Vo.l 9 No. 1 2011 1 Ch inese Journal of B ioprocess Engineering Jan. 2011 do:i 10. 3969/ .j issn. 16 2- 36 8. 2011. 01. 00 L- 柴进凯, 张伟国 (江南大学 工业生物技术教育部重点实验室, 无锡 214122) : 以嗜乙酰乙酸棒杆菌( Corynebacterium acetoac idop h ilum ) LG-3 为出发菌株, 经紫外线 ( UV )和硫酸二乙酯 ( DES) 诱变处理,磺胺胍( SG)高浓度( NH ) SO 定 筛选, 获得 1株谷氨酰胺高产菌株 LG-65, 在未经优化的条件 4 2 4 下摇瓶发酵 2 h可产谷氨酰胺 4315 g/L, 比出发菌株的产量 3214 g/L提高了 341 3% 在此基础上, 对其发酵条件 进行优化, 经 2 h摇瓶发酵产量可达 4 ~ 48 g /L, L 发酵罐补料分批发酵 40 h可达 55 g /L : 育种; 发酵; L-谷氨酰胺; 诱变 : Q815 : A : 16 2- 36 8( 2011) 01- 0029- 06 Breeding ofL-glutam ine high producing strain and its fermentation conditions CHA I Jinka,i ZHANG W eiguo ( Key Laboratory of Industrial B iotechnology of theM in istry of Education, Jiangnan Un ivers ity, Wuxi 214122, Ch ina) Abstract: The original strain LG-3 w as mutated by ultraviolet rad iation and d iethyl su lfate, and screened in culture dish having high concentration of sulfaguan id ine or ( NH ) SO . A strain LG-65 w as obtained 4 2 4 for producing 4315 g /L of L-glutam ine. The fermentation cond itions w ere further optmi ized, the yield reached 4 - 48 g/L in shaker flask for 2 h and reached 55 g/L cultivated in L fermentor for 40 h. Key words: breeding; fermentation; L-glutam ine; mutation [ ] L-(L-glutam ine, L-G ln)L- 1 000 t , L-G ln C-