神经肽Y对心室肌细胞离子通道的影响.pdfVIP

生理学报 Acta Physiologica Sinica, June 25, 2006, 58 (3): 225-231 225 研究论文 神经肽 Y 对心室肌细胞离子通道的影响 赵画晨，刘忠保，封启龙，崔香丽，张春敏，吴博威* 山西医科大学生理学教研室，太原030001 摘 要：采用全细胞膜片钳技术观察神经肽Y (neuropeptide Y, NPY)对心室肌细胞离子通道的影响。结果如下：(1) NPY 浓 度在1.0~100 nmol/L 范围内剂量依赖性抑制大鼠心室肌细胞ICa-L ，IC50 值为1.86 nmol/L。NPY 对ICa-L 的I -V 曲线的最大峰值 电位、激活和失活电位均无显著影响。NPY 对去甲肾上腺素(norepinephrine, NE)增加的I Ca-L 有显著抑制作用。(2) NPY 对 大鼠心室肌细胞INa/Ca 有显著抑制作用。10 nmol/L NPY 使前向INa/Ca 由(0.27 ±0.11) pA/pF 减小为(0.06 ±0.01) pA/pF；反向 INa/Ca 由(0.45 ±0.12) pA/pF 降为(0.27 ±0.09) pA/pF (P0.05 ，n=4) 。(3) NPY 对大鼠心室肌细胞Ito 有显著增强作用。10 nmol/L NPY 使Ito 由(12.5 ±0.70) pA/pF 增加至(14.7 ±0.59) pA/pF (P0.05 ，n=4) 。(4) 10 nmol/L NPY 对大鼠心室肌细胞INa 没有显著影响。(5) 10 nmol/L NPY 对豚鼠心室肌细胞I K 无明显影响。研究结果证实，NPY 抑制大鼠心室肌细胞I Ca-L 和INa/Ca ， 增强I to ，对I Na 和豚鼠心室肌细胞I K 没有显著作用，表明NPY 对上述主要离子通道的效应与NE 的效应相拮抗。 关键词：神经肽Y ；膜片钳技术；心肌；离子通道 中图分类号：Q 4 6 3 Effects of neuropeptide Y on ion channels in ventricular myocytes ZHAO Hua-Chen, LIU Zhong-Bao, FENG Qi-Long, CUI Xiang-Li, ZHANG Chun-Min, WU Bo-Wei* Department of Physiology, Shanxi Medical University, Taiyuan 030001, China Abstract: Neuropeptide Y (NPY) co-exists with norepinephrine (NE) in sympathetic terminals, and is the most abundant neuropeptide in myocardium. Many studies have focused on the effects of NE on ion channels in cardiac myocytes and its physiological significance has been elucidated relatively profoundly. There have been few investigations, however, on the physiological significance of NPY in 2+ myocardium. The effects of NPY on L-type Ca channel currents (ICa-L) were evaluated in some studies and different results were presented, which might be attributed to the different species of animal tested and different methods used. It is necessary, therefore, to study the effects of NPY on ion channels in cardiac myocytes systematically and furthe