银杏叶提取物抗猪血清致大鼠免疫性肝纤维化作用的实验研究.doc

银杏叶提取物抗猪血清致大鼠免疫性肝纤维化作用的实验研究 韦建乔1 谭忠谋2 （1.主管药师，广西河池食品药品检验所，广西，河池，547000；2，副主任药师，广西河池食品药品检验所，广西，河池，547000） 摘要：目的 研究(EGb)对所致大鼠肝纤维化的保护作用。方法 血清中谷草转氨酶(AST)、谷丙转氨酶(ALT)(LN)及(HA)，结果 能明显降低所致ALT、AST(P 0.05或P0.01)，明显肝组织纤维化变性。结论 对所致大鼠肝纤维化具有保护作用。 关键词 Experimental Study of EGb on Rat Immunological Hepatic Injury Induced by procine Serum Abstract:Objective To investigate the protective effects of EGb on procine serun (PS)-induced immunological hepatic fibrosis in rats. Methods The immunological hepatic fibrosis model was induced by intraperitoneal injection of pig serum．respective dose drug once daily for 4 consecutive weeks. 24 hours after the last administration of drugs, all the animals were sacrificed, and the blood serum and liver tissue were taken quickly.The ativity of AST, ALT and the concentration of LN , HA in serun were measured.Moreover, histopathological observation was assayed for the degree of hepatic fibrosis. Result Compared with the model control group, in all doses of EGb groups and positive control group, the activities of AST , ALT and the concentrations of LN, HA in serum were decreased significantly（P0.05 or P0.01).And the degree of hepatic injury in high dose and middle dose EGb groups and positive control group could be lessened (P0．05 or P0.01). Conclusion Our results show that EGb has significant protective effects on procine serum-induced immune hepatic fibrosis in rats． Keyword: ginkgo biloba extract immunological hepatic fibrosis rats procine Serum 肝纤维化是各种慢性肝炎共同的病理基础， 也是肝硬化必经的病理过程(1)。大量研究表明肝纤维化是可逆的,但临床上仍缺乏有效的抗肝纤维化药物。银杏叶提取物(EGb)是从银杏科植物银杏的叶子中提取分离纯化的物质，主要活性成分是黄酮类和萜内酯类(2)。现代药理学研究表明EGb具有免疫调节，拮抗血小板活化因子，改善微循环，抗氧化，降血脂，抗动脉硬化等作用(3-7)。临床上主要用来治疗心脑血管方面的疾病，如高血压、高脂血症、冠心病、心绞痛、脑血管疾病等(8)。银杏叶提取物对非酒精性脂肪性肝炎肝损伤有较好的抑制作用(9)，对四氯化碳引起的肝纤维化也具有良好的治疗作用(10)。目前，银杏叶提取物对猪血清引起的大鼠免疫性肝纤维化的影响仍未有人研究。本实验拟用猪血清诱导的肝纤维化模型研究EGb对免疫性肝纤维化的影响，为临床合理应用EGb提供理论依据。 1材料与方法 1．1材料 1．1．1 实验动物 SPF级的SD大鼠，雌雄各半，体重180-220g，，购于广西医科大学实验动物中心。生长环境为恒温 (22～2)℃，相对湿度45％～50％ 1．1．2试剂 猪血清，秋水仙碱，银杏叶提取物(含黄酮