采用响应面设计法优化光合细菌培养基.docVIP

采用响应面设计法优化光合细菌培养基 摘要:本试验采用响应面设计法优化光合细菌培养基。结果表明:培养基成分中醋酸钠和蛋白胨对于光合细菌的生长影响最为显著,最优培养基配方为:醋酸钠1. 145 g/L、蛋白胨0. 055 g/L、碳酸氢钠0. 6 g/L、硫代硫酸钠0. 4 g/L、氯化钠0. 3 g/L、硫酸镁0. 1 g/L、磷酸二氢钾0. 05 g/L。在此条件下,光合细菌生长最为良好,经过5 d培养以后,培养液OD600可以达到0. 5以上。 关键词:光合细菌;培养基;优化;响应面方法 Optim ization ofCultureM edium for Photosynthetic Bacteria by Response SurfaceM ethodology Abstract:Response surfacemethodologywas used to optimize the culturemedium forPhotosynthetic bacteria(PSB).The resultshowed thatsodium acetate and peptone has themostsignificant influence on PSB cultures.The optimizedmedium forPSB culturewas as follows: sodium