-MetabolomicsAnalysisofSaccharomycescerevisiaeby：-代谢组学酿酒酵母的分析.pdfVIP

Metabolomics ASMS 2009 Analysis of Stefan Jenkins¹ ², Sally Webb¹, Saccharomyces Theodore R. Sana¹ ¹Agilent Technologies, Santa Clara, CA, cerevisiae by LC/MS- USA ²Cell and Molecular Biology TOF Using a Robust MS Program, San Francisco State University Milling Protocol for WPD 114 Extraction Metabolomics Analysis of Saccharomyces cerevisiae by LC/MS-TOF Using a Robust Milling Protocol for Extraction Introduction Experimental Baker’s yeast, Saccharomyces cerevisiae, is extensively Initial yeast culture used as a model organism for all eukaryotic cells, facilitating research into the biochemistry and biological pathways of more complex but closely related mammalian cells. Metabolomics represents a quickly growing component in the field of systems biology. It entails the Centrifuge and PBS wash study of an organism’s metabolome, or complete set of small molecule metabolites comprised of a wide variety of Quench with compounds. A robust controlled protocol is required for MeOH:PBS studying particular pathways, especially when comparing Add external different strains of yeast. We have tested an optimized standards