EbashiSCalciumbindingactivityofvesicularrelaxingfactor：江桥的囊泡的松弛因子的钙结合活性.pdfVIP

CC!NUMflER 44 __ This Week’s Citation Classic ® Ebashi S. Calcium binding activity of vesicular relaxing factor. J. Biochern. Tokyo 50:236-44. 1961. [Rockefeller Institute. New York. NY] This paper offered a quantitative basis or the Ca logical relaxing factor must also bind Ca very concept for muscle contraction, a concept that at that strongly and the relaxation must be brought about time had not been accepted by the majority. The bythe removal of essential Ca from the actomyosin 1~ relationship between Ca- concentration and the system. contraction of the actomyosin system had been exten- The experimental results were just what I had 1 sively studied. Natural actomyosin, which had been expected, which, in itself, was unusual because my deprived of most of its contractility as a result of earlier predictions had never been correct. I still careful washingto eliminate contaminating Ca, could well remember the day early in June 1959 when I 2~ be fully activated by a few pM Ca significant, 2~ acti- conducted the first experiment and found that the vation being seen even with 0.2 pM Ca This. was relaxing factor beautifully bound Ca inthe presence 2~ the first indication of a physiologically effective Ca of ATP. I triumphantly told Professor Lipmann, ex- concentration, which was later revealed to be com- pecting his praise, but I soon realized that I