FigureS1ThewildtypeCHOcells：图1野生型首席人事官细胞.pdfVIP

Electronic Supplementary Material (ESI) for Medicinal Chemistry Communications This journal is © The Royal Society of Chemistry 2011 1 2 3 Figure S1. The wild type CHO cells do not adhere to collagen I, and neither do CHO-α2 or 4 CHO-wt cells adhere to plastic. 5 6 Electronic Supplementary Material (ESI) for Medicinal Chemistry Communications This journal is © The Royal Society of Chemistry 2011 1 Materials and Methods 2 Molecular modeling 3 All molecules during the development process were sketched by using Sybyl 7 (Tripos Inc., St. Louis, MO, USA), and energy 1 4 minimized using the Merck molecular force field (MMFF94) and conjugate gradient method until the energy gradient was less 2 5 than 0.05 kcal/mol. Energy minimized molecules were docked to the closed conformation of α2I domain with Gold3.1.1. using 6 search area of a 15 Å radius sphere centered at the Mg2+ ion. Gold was selected for this study, because the scoring function has 7 been developed by using the small molecule crystal structure data, and the metal coordination geometries are properly described. 8 Protein structures were acquired from the PDB 3. Hydrogen atoms were added to the protein structure with Sybyl. Protonation of 9 histidines was selected on the basis of hydrogen bonding with nearby atoms. All docking results were visually inspected by using 4 10 Bodil Modeling Enviro