organculturemechanismofaction：器官培养机制的作用.pdfVIP

Journal of Ethnopharmacology 97 (2005) 397–403 Effect of Diabecon on sugar-induced lens opacity in organ culture: mechanism of action a b b a,∗ M.S. Moghaddam , P. Anil Kumar , G. Bhanuprakash Reddy , V.S. Ghole a Biochemistry Division, Department of Chemistry, University of Pune, Pune 411007, India b National Institute of Nutrition, Hyderabad 500007, India Received 21 June 2004; received in revised form 2 October 2004; accepted 30 November 2004 Available online 18 January 2005 Abstract Cataract is the leading cause of blindness worldwide. Apart from ageing, diabetes has been considered to be one of the major risk factors of cataract. The high sugar levels in diabetes may cause tissue disruption and intumescences by osmotic changes induced via aldose reductase (AR) mediated polyol pathway. Therefore, agents that can inhibit AR and prevent sorbitol accumulation may be helpful to combat sugar- induced cataract. In the present study, AR inhibitory activity of Diabecon (an herbal drug used for diabetes) was studied together with its effect against sugar-induced lens opacity in organ culture. Diabecon aqueous extract (DAE) showed potential inhibitory activity with an IC50 value of 10 g/ml against rat lens AR. Incubation of goat lens with supraphysiological concentrations of glucose (100 mM) led to the loss of lens transparency associated with increased AR activity, decreased soluble protein and increased protein carbonyls and glycation. Addition of DAE (0.3 mg/ml) to the medium preserved transparency and ameliorated the