ProductionandApplicationofLaccaseEnzymeinPulpand：生产和浆漆酶的应用.pdfVIP

IMPACT: International Journal of Research in Applied, Natural and Social Sciences (IMPACT: IJRANSS) ISSN(E): 2321-8851; ISSN(P): 2347-4580 Vol. 2, Issue 4, Apr 2014, 153-158 © Impact Journals PRODUCTION AND APPLICATION OF LACCASE ENZYME IN PULP AND PAPER INDUSTRY 1 2 SUKHBIR KAUR VARSHA NIGAM 1Department of Microbiology, Guru Nanak Khalsa College, Yamuna Nagar, Haryana, India 2Department of Botany, Guru Nanak Khalsa College, Yamuna Nagar, Haryana, India ABSTRACT Laccase is an enzyme that has potential ability of oxidation. There are diverse sources of laccase producing organism like fungi, plants and microorganism. The possibility of using crude laccase in the dechlorination of chlorine-based bleached kraft hard wood pulp was investigated. The present work comprises the laccase enzyme production by isolated ligninolytic fungal strain SL and SL and its industrial application. Experiment was conducted on 2 4 bleaching of kraft hard wood pulp with laccase enzyme (122.33 IU/ml) produced by ligninolytic fungal strain SL which 4 shows brightness, whiteness and improvement along with CLO reduction. The addition of laccase inducer Cuso4 in to the 2 culture medium led to an increase dechlorination activity. KEYWORDS: Kraft Pulp, Laccase, Dechlorination, Biobleaching INTRODUCTION