白血病G6PD蛋白表达的研究.pdfVIP

大理学院学报 第10卷第2 期 2011年2 月 JOURNAL OF DALI UNIVERSITY Vol.10 No.2 Feb. 2011 白血病G6PD蛋白表达的研究 * 蒋 剑，李永萍，潘 云，李正金，王红霞 （大理学院附属医院，云南大理 671000） ［摘要］目的：观察白血病患者骨髓中白血病细胞葡萄糖-6磷酸脱氢酶（G6PD）蛋白的表达情况，探讨G6PD与白血病的关系。方 法：应用免疫组化方法检测35例白血病患者和35例非恶性血液病患者骨髓组织标本中白血病细胞G6PD蛋白的表达，比较白血 病患者和非恶性血液病患者G6PD蛋白表达率的差异。结果：G6PD蛋白在白血病骨髓组织白血病细胞中呈现弱阳性表达。35例 白血病患者骨髓组织中G6PD蛋白阳性表达率为25.7% （9/35），而35例非恶性血液病患者骨髓组织中G6PD蛋白阳性表达率为 2.9% （1/35），差异有统计学意义（P 0.05）。结论：白血病可能存在G6PD蛋白的表达上调，值得进一步研究。 ［关键词］G6PD蛋白；白血病；免疫组化 ［中图分类号］R733.7 ［文献标志码］A ［文章编号］1672-2345 （2011）02-0027-03 Expression of G6PD Protein in Leukemia * JIANG Jian, LI Yongping , PAN Yun, LI Zhengjin, WANG Hongxia （Affiliated Hospital of Dali University, Dali, Yunnan 671000 ，China） 〔Abstract 〕Objective: To investigate the expression of G6PD protein in human leukemia and analyze the relationship between G6PD and leukemia. Methods: Immunohistochemical method was applied to detect the expression of G6PD protein in the bone marrow tissue of 35 leukemia patients and 35 cases of non-hematologic malignancies. Non-hematologic malignancies were used as control group. The comparison of G6PD protein rate in leukemia patents and the control was studied. Results: The positive expression rate of G6PD protein was 25.7% （9/35）in leukemia patients and 2.9% （1/35）in control. Data showed a statistically significant difference in G6PD expression between the two groups （P 0.05）. Conclusion: G6PD protein was high expressed in leukemia, which needed further study. 〔Key words 〕G6PD protein; leukemia