大黄对大鼠腹腔感染致肺损伤的防治作用.pdfVIP

198 Ch na Pharmac st 2003, V ol. 6 No. 4 ## 束长辉 朱义用 ( 解放军第101 医院 无锡214044) 景炳文 ( 上海长征医院急诊科) 娄永华 ( 第二军医大学微生物教研室) : 探讨大黄对大鼠腹腔感染致肺损伤防治作用的机 : 采用大鼠盲肠结扎并穿孔( CLP ) 造成腹腔感染, 每天在麻醉下经胃管灌注大黄 1 次分别在术后0 , 24, 48, 72, 96, 120 h 检测肺毛细血管通透性肺组织的内毒素和肿瘤 坏死因子( T N F-A) , 并取支气管肺泡灌洗液( BAL F) 进行细胞学分析: 肺毛细血管通透性BA LF 的中性粒细胞百分率 肺组织的内毒素和T N F-A 逐渐增加, 时间越长越明显, 但药物组比感染组增加较慢幅度较小: 大黄可防止内毒素进 入肺组织, 抑 肺内中性粒细胞积聚, T N F-A释放, 减轻肺的炎性反应 大黄 肺损伤 肺毛细血管通透性 中性粒细胞 内毒素 肿瘤坏死因子 : R2 85- 5 : A : 100 8- 049X( 200 3) 04-0 19 8- 0 3 Prophylactic Effects of Rhubarb on Lung Injury Caused by Abdominal Infection in Rats Shu Changhu , Zhu Y y ong ( 10 1st Hosp tal of PLA , Wux J angsu 2 14044 P . R. Ch na) J ang B ngw en ( Department o f Emergency M ed c ne, SHangha Chang Zheng Hosp tal) L ou Y ong hua ( Department of M crob ology, the 2nd M l tary and M ed cal U n vers ty) ABSTRACT Objecti e: T o study the underly ng mechan sms of the prophylact c eff ects n rhubarb on early lung njury caused by the abdom nal nf ect on n rats. Method: T he cecal l g at o n and perforat on ( CL P) w as adopt to make a abdom nal nf ect on n rats. R hubarb w as njected nto the stomaches of rats through tubes once a day . T he subg roups of rats w er e sacr f ced at 0 , 24 , 48, 72 , 96 and 120 hours after o perat on respect vely . T he permeab l t y of pulmonary cap llary, the concentrat on of endotox ns, and tumor nec- cros s factor-A( I FN-A) n lung w ere assayed. T he cytolog cal analys s for bro nchoalv elar lavage flu d( BA LF ) w as perfomed . Result: T he permeab l ty of pulmonary cap llary, the neutroph l percentage of BAL F, and the concentrat on of endotox ns and T N F-A n lung ncreased gradually, the more t me the mor e obv