食子酸酯对人肝细胞氧化损伤的保护作用.pdfVIP

ofAnhui 安徽农业科学。Journal A班．Sci．2009，37(6)：2561—2563 赍任编辑李菲菲责任校对傅真治 表没食子儿茶素没食子酸酯对人肝细胞氧化损伤的保护作用 苏畅，刘银花，卢章显 (韶关学院医学院药学系，广东韶关512026) 含量显著升高。与模型组相比，EGCGl．25×10一、2．50×lo一、5．00 著降低，且EGCG 1．25×10。和 2．50 x10。mol／L刹量组可使SOD活性极显著增加，EGCG5．00×104和1．00×10‘4 tool／L剂量组可极显著降低MDA含量。[结论] EGCG可减轻CCl。及H：O：所致的肝细胞氧化损伤，对肝细胞具有保护作用，并具剂量依赖性。 关键词茶多酚；EGCG；CCl,；H，O，：抗氧化 文献标识码A 中图分类号$567．1+9 文章编号晒17—661l(2009)06—0256l一03 Protectionof onHumanLiverCellsfromOxidative Epigallocatechin-3-gallate Injury SU etal Chang (PharmaceuticalDepartment，MedicalCoUege，ShaoguanUniversity，Shaoguan，Guangdong512026) aim Abstract 111e waBtodiscussthe humanlivercells(L02)from fObjectivel protectionofepigallocatechin-3．gaUate(EGCG)oni．jury． cellsurvival content8of and MDA Method】Throughdetermining rate，the transaminases(ALTAST)，lactate andthe ofSODinthecellculture ofEGCGonL02from was with activity supematant。theprotection i．jury researched．[ResultCompared thecontroled themodel with cell ofSODwagdecreased contentofMDAwagin． group，in group liver i．jury，theactivity significantly。the creased dose content thecontentsofALTandLDH tIle ofEGCGcould decreasethe ofMDAand extremelysignificantly．allgroups significantly wereincre∞ed initsculture withthemodel contentsofALTweredecreased inthe significantly superrmtant．Comparedgroup．the significandy ceilculture ofthe X10一’and1．OO×10—4mol／LofEGCGandthecontentsofLDH supernatantsdosegroups1．25×101’．2．50×10—3。5．00