T淋巴瘤EL_4细胞双受体表达研究.pdfVIP

404 201127 doi: 10. 3969/ j. issn. 1000-484X. 2011. 05. 005 T EL-4 ( 南方医科大学生物技术学院分子免疫学研究所, 广州510515) R392. 12 A 1000-484X( 2011) 05- 0404-05 [ ] : T EL-4 T (T ell re eptor,TCR) , T : , EL-4 mRNA, DNA, RT-PCR 24 TCR BV CDR3 , ( GeneS an) , TCR BV CDR3 : 24 BV , T CR BV CDR3 ( Gaussian distribution) , 24 BV EL-4 TCR BV10 BV12 , CDR3 , RT-PCR TCR , : EL-4 TCR, TCR T [ ] EL-4 ;T (TCR) ; ; Expression of two distinct T ce receptors by EL-4 T ymphoma ce s ZHANG Yuan, LUO Wei, MA Li. Institute of Molecular Immunology, School of iotechnology, Southern Medical Universi- ty, Guangzhou 510515, China [Abstract] Objective:To investigate the expression of T ell re eptor (TCR) inEL-4 T lymphoma ells. Methods:Mouse spleen ells were used as positive ontrol, total RNA was isolated from mouse spleen ells and EL-4 ell line. Complementarity Determining Region 3 ( CDR3) of 24 TCR BV gene subfamilies was amplified by the reverse trans ription polymerase hain rea tion ( RT-PCR) . To determine the spe tratypes and sequen es of CDR3 region of TCR BV subfamilies expressed by EL-4, PCR produ ts were analyzed by TCR GeneS an T e h- nique and DNA sequen er, respe tively . Resu ts: 24 TCR BV subfamilies of mouse spleen ells were poly lonal, the spe tratypes of TCR CDR3 region were showed Gaussian distribution. EL-4 was shown dete ted the expression of TCR BV10 and BV12 simultaneously, spe tratypes of whi h showed single peak, and the TCR sequen es of BV10 and BV12 were onfirmed by DNA sequen er and they were both in-frame rear- rangements. Conc usion:Two sets of in-frame rearrangements of TCR hain exist in EL-4, and there maybe defe ts in alleli ex lusion in TCR hain. It provides a basis for future resear h of alleli ex lusion me hanism. [Key words] EL-4 ell line;T ell re eptor (TCR) ;Defe t in alleli ex lusion; In-frame rearrangement T