保肝宁对肝纤维化的相关影响.docVIP

保肝宁对肝纤维化的相关影响 摘要：目的；探讨保肝宁抗肝纤维化的作用机制。方法：采用复合因素建立肝纤维化大鼠模型, 采用全自动生化分析仪检测总胆固醇（TC）、甘油三脂（TG, 采用同位素放射免疫分析法检测血清瘦素（Leptin）水平。观察保肝宁对肝纤维化模型大鼠TC、TG及Leptin水平等各项指标的影响。结果：与正常组组比较, 模型组及各药物组血清TC、TG, 差异有非常显著性意义（P0.01）； 与模型组比较, 保肝宁大、中剂量药物组血清TC、TG水平明显升高, 差异有非常显著性意义（P均0.01）。其中, 保肝宁大剂量升高TC、TG作用明显明显优于秋水先碱组(p0.05)。与正常组比较, 模型组及各药物组血清水Leptin平均明显升高(P均0.05)。与模型组比较, 各药物组血清Leptin水平明显下降, 差异有显著性意义(P0.01,P0.05) , 各药物组降低Leptin作用, 差异无显著性意义(P0.05)。结论：保肝宁可有效防治大鼠肝纤维化, 其作用机制可能与通过改善肝功能, 调节血脂, 降低血清瘦素水平密切相关。 关键词：肝纤维化 模型大鼠 中医药疗法 保肝宁 血脂瘦素 Effect of Chinese medicine Baoganning liver fibrosis model rats Abstract: Objective:To discuss the mechanism of Baoganning anti-liver fibrosis.Methods: Liver librosis rat models were established by complex factors.Automatic biochemical analyzer was used to detect serum leptin.To observe the influence of Baoganning on TC,TG and leptin level of liver fibrosis model rats.Results:Compared with the normal group,level of serum TC,TG decreased apparently in model and every drug groups(P0.01).Compared with the model group,level of serumTC,TG increased apparently in high and medium doseof Baoganning groups(P0.01).The effect of high dose of Baoganning on advancing TC,TG was better than that of colchicin(P0.05).Compared withnormal group,the level of serum leptin of model group and each drug group increased significantly(P0.01).Compared with model group, the level of serum leptin of Baoganning groups decreased significantly(P0.01,P0.05).There was no significant differences among every drug group in lowering leptin (P0.05).Conclusion:Baoganning can effectively prevent andtreat liver fibrosis in rats ,whichmay be closely related to the improvement of liver function,adjustment of lipid and reduction of serum leptin. Key words:Liver fibrosis Model rats TCM therapy Baoganing;Lipid Leptin 肝纤维化（hepatic fibrosis）是由于纤维增生和纤维分解不平衡,导致肝脏纤维结缔组织的过度沉积所致，是各种慢性肝病后产生的一种共同结果，同时，也是一切慢性肝病的病理学基础。瘦素是肥胖基因编码的肤类激素，同时具有细胞因子特性，它具有许多重要的生理作用，包括炎症放大作用和免疫功能。研究显示：瘦素与肝纤维化的形成之间有着密切关系。对其作用机制的深人研究也许是揭示肝纤维化形成机制的新的切