人参拮抗高皮质激素血症致神经元损伤的作用研究.pdfVIP

0 [1] [2] [3~4] [5] [7 8] [6] [7] GluKA [8] 1 1.1 C57BL/6 18~22g SCXK 2007-0005 12 h Panax ginseng C. A. MeyCorticosterone sigma β-actin SYP SantaCruz NF-LInvitrogen BDNF GDNFSanta Cruz HRP IgG IgG Gene Script 1.2 1.2.1 Zhao [9] DMSO 20 mg·kg-1 1.2.2 30 min 20~30 min 3 g ·ml-1 3 7.2g·kg-13.6 g·kg-11.8 g·kg-13.6 mg·kg-1 1.2.3 6 8 4 4 western-blot NF-LSYP BDNFGDNF 1.2.4 western-blot 1mL RIPA 50 mM Tris-HCL 0.1%SDS 1%NP-40 1mMEDTA150 mM NaCl1 mM PMSF1 μg·mL-1 1 μg·mL-1 PH