e6 微生物学-南京师范大学 英文版PowerPoint课件.pptVIP

summarize 1）Embden-Meyerhof-Parnas Pathway (EMP) (Glycolysis, Hexose Diphosphate Pathway) glucose pyruvate with O2 ：connecte EMP pathway with TCA pathway； without O2：reduce some metabolism product, only energy-yielding process. generates ATP by substrate-level phosphorylation： （1） glyceraldehyde 1,3-phosphate ? 3-phoshoglyceric acid + ATP； （2） PEP ? pyruvate + ATP fructose 6-phosphates： be converted to glucose 6-phosphates ，be returned to Pentose Phosphate Pathway glyceraldehyde 3-phosphate ： a. through EMP pthway， be converted to pyruvate，into TCA pthway b. converted to Hexose Phosphate, be returned to Pentose Phosphate Pathway The overall reaction： 6 glucose 6-phosphates +12NADP++3H2O → 5 glucose 6-phosphates + 6CO2+12NADPH+12H++Pi 4) phosphoketolase pathway (PK) a.Pentose phosphoketolas Pathway G xylulose 5-phosphate G fructose 6-phosphates 1. definitions broader ：use microorganism to produce useful metabolic product narrower：under anaerobic conditions, be defined as an energy-yielding process using itself metabolic intermediates as the final hydrogen (electron) accepter organic molecules serve as both electron donors and acceptors. trait： 1） generates ATP by substrate-level phosphorylation； 2）the glucose is partially oxidized ，mostly energy in fermention products ； 3）lower energy； 4） generates many kinds of fermention products b. bacteria (Zymomonasmobili, Pseudomonas saccharophila ) homoalcohol fermentation 1 G 2 pyruvate 4) acetone-butanol fermentation （ mixed, acetone:butanol : ethanol = 3：6：1） ——Clostridium acetobutyricum The Stickland reaction is used to oxidize several amino acids: alanine, leucine, isoleucine, valine, phenylalanine, tryptophan, and histidine. Bacteria also ferment amino acids (e.g.,