哺乳动物硒蛋白地研究进展.pdfVIP

1998 29 1 29 张在香 杨晓光 夏弈明 陈孝曙( ,100050) 硒是哺乳动物和人必需的微量元素硒的生物学功能主要是以硒蛋白的形式 表现的到目前 止,已经克隆并测定cDNA 顺序的哺乳动物硒蛋白有9 种, 它们是 细胞内谷胱甘肽过氧化物酶细胞外谷胱甘肽过氧化物酶磷脂氢谷胱甘肽过氧化物 酶胃肠谷胱甘肽过氧化物酶型碘化甲状腺原氨酸5脱碘酶型碘化甲状腺原 氨酸5脱碘酶型碘化甲状腺原氨酸5脱碘 酶硒蛋白P 和硒蛋白W这些硒蛋 白中硒参入到蛋白分子是通过硒半胱氨酸-tRNA 识别mRNA 中特异的UGA 密码 子将硒半胱氨酸插入到硒蛋白中 硒;硒蛋白;生物功能;表达调控 Progress in the Study of Mammalian Selenoprotein ZHANG Za-i Xiang, YANG Xiao- Guang, XIA Y-i ing, CHEN Xiao-Shu ( I nstit ute of N ut ri tion and Food H yg iene, Chi nese A cademy of Prev ent iv e M ed icine, Beijing 100050) Abstract Selenium is an essential trace element for animals and humans. Selenium ex- erts its biological function largely through selenoproteins. Up to now nine selenopro- teinscDNAs have been cloned and sequenced, which are cellular glutathioneperoxidase ( cGPX), extracellular glutathine peroxidase( eGPX), phospholipid hydroperoxidase glutathione peroxidase( PHGPX), gastrointestinal glutathione peroxidase ( GPX-GI) , type iodothyronine 5-deiodinase( ID ), type 2 iodothyronine 5-deiodinase( ID) , type iodothyronine 5-deiodinase( ID ), selenoprotein P ( Se-P) and selenoprotein W. In all these selenoproteins Se is incorporated into the protein molecule via the se- lenocysteiny-l tRNA which recognizes the specific UGA codons in mRNA to insert se- lenocysteine into the primary structure of selenoproteins. Key words Selenium; Selenoprotein; Biological function; Expression and regulation (Se) 1817 Berzelius 1957 Schwarz Foltz Se ,,9 cD- [1] NA , 9 , , 10 1973 Hoekstra Rotruck , 30 1998 29 1 , ( cGPX) , ,cGPX ( eGPX) 70 cGPX [ 2] 1985 Cohen 1987 Takahashi , cGPX, (eG