茉莉酸信号转导突变体ber15的分离与基因克隆表明油菜素内酯的合成影响茉莉酸信号转导.pdfVIP

植物学通报 2006, 23 (5): 603~610 Chinese Bulletin of Botany 研究论文 茉莉酸信号转导突变体ber15 的分离和基因克隆表明油菜素 内酯的合成影响茉莉酸信号转导 1,2 3 1 1* 郑文光 ,耿宇 ,李常保 ,李传友 1 中国科学院遗传与发育生物学研究所, 北京 100101 2 中国科学院研究生院, 北京 100039 3 广西大学园艺系, 南宁 530004 摘要 bestatin是一种氨肽酶抑制剂, 能够激活茉莉酸信号转导途径而诱导抗性相关基因的表达, 从而为 用化学遗传学手段解析茉莉酸途径提供了一个有效的工具。ber15是我们鉴定到的一个对bestatin不敏感 的拟南芥突变体, 随后的研究表明该突变体对外源茉莉酸的敏感性也明显降低, 表明相应的野生型基因 BER15在茉莉酸信号转导中起重要作用。图位克隆结果表明BER15编码一个细胞色素P450单加氧酶, 是 植物激素油菜素内酯合成途径中的一个关键酶。对BER15基因功能的深入研究将会为了解油菜素内酯的 合成与茉莉酸信号途径间的互作关系提供证据。 关键词 茉莉酸, bestatin, 油菜素内酯, 化学遗传学, 信号互作　 Characterization of Jasmonic Acid Response Mutant ber15 Demonstrates Cross Talk Between Jasmonic Acid and Brassinosteriod Signaling Wenguang Zheng 1, 2 , Yu Geng 3 , Changbao Li 1 , Chuanyou Li1* 1Institute of Genetics and Developmental Biology, Chinese Academy of Sciences , Beijing 100101 , China 2 Graduate School of Chinese Academy of Sciences, Beijing 100039 , China 3Department of Horticulture, Guangxi University , Nanning 530004 , China Abstract Bestatin, an inhibitor of some aminopeptidases, potently activates jasmonic acid-induced defense genes expression in the model systems of tomato and Arabidopsis. We have employed bestatin as a small molecular chemical tool to dissect jasmonic acid signaling in Arabidopsis and identified a collection of bestatin response (ber) mutants. ber15 is one of these mutants that are resistant to the inhibitory effect of bestatin on root elongation. Subsequent investigation revealed that ber15 is less sensitive to applied jasmonic acid in term of root growth inhibition, suggesting that the BER15 gene play a role in JA signaling. Map-based cloning of BER15 demonstrated BER15 encodes a cytochrome P450 monooxygenase which acts as a key enz