紫蘑菇多糖的提取和体外抗肿瘤活性研究.pdfVIP

, J urnal f Anhui Agri. Sci. 2008, 36(36) : 15955- 15957 王淼 张士敏 * 葛淑敏, 于源华 , 张艳飞 (, 130022) [ 目的] 试验优化了紫蘑菇多糖的提取条件, 探讨了紫蘑菇多糖对人宫颈 细胞肝 细胞的增殖抑制作用[ 方法] 采用正交试 验,MTT 比色法测定[ 结果] 试验成功提取了紫蘑菇多糖, 试验结果显示紫蘑菇多糖对人宫颈 细胞肝 细胞均具有一定的抑制作 用[ 结论] 试验为真菌多糖的提取及抗肿瘤活性研究奠定了基础 紫蘑菇多糖; 提取; 人宫颈 细胞; 肝 细胞; 抑制 + S567. 3 9 A 0517- 6611( 2008) 36- 15955- 03 Study on the Extraction of Polysaccharide from Agaricus rubell us and Its Antitumor Activity in vitro GE Shumin et al ( Department f Bi techn l gy, Sch l f Life Science and Techn l gy f Changchun University f Science and Techn l gy , Changchun, Jilin 130022) Abstract [ Objective] The test aimed t ptimize the extracti n c nditi ns f p lysaccharide fr mA . rubellus and discuss the inhibit ry effect f p lysac charide fr m A . rubellus n the pr liferati n f human cervical cancer cells and liver cancer cells. [ Meth d] The rth g nal experiment and MTT c l rimetry assay were made in the study. [ Result] P lysaccharide was successfully extracted fr mA . rubellus. The test results sh wed that p lysaccharide fr mA . rubellus had certain inhibit ry effects n human cervical cancer cells and liver cancer cells. [ C nclusi n] The test laid the f undati n f r studying the extracti n f fungi p lysaccharides and the antitum r activity. Key words P lysaccharide fr m A . rubellus; Extracti n;Human cervical cancer cells; Liver cancer cells; Inhibiti n ( Agaricus rubellus) , 1. 2.2. 1 COP , , 9 , 1 , , , 30 ml , 60 [ 1- 2] ! 3 h, , , , , ( purple , mushr m p lysaccharides, COP) , MTT 1 COP (Hela) Table 1 The ortho onal experimental desi n for the de reasin conditions (HepG2) ∀% ∀! ∀h 1