Lithium Chloride Inhibited Neuronal Apoptosis and Glycogen Synthase Kinase -3β Activities in Hippocampal CA1 Area After Middle Cerebral Artery Occlusion and Reperfusion in Rats.pdfVIP

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Lithium Chloride Inhibited Neuronal Apoptosis and Glycogen Synthase Kinase -3β Activities in Hippocampal CA1 Area After Middle Cerebral Artery Occlusion and Reperfusion in Rats.pdf

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Lithium Chloride Inhibited Neuronal Apoptosis and Glycogen Synthase Kinase -3β Activities in Hippocampal CA1 Area After Middle Cerebral Artery Occlusion and Reperfusion in Rats.pdf

Xin—heWang,Qing—mingBian，JieSun，Yan—ningQian DepartmentofAnesthesiology，TheFirstAffiliatedHospitalofNanjingMedicalUniversity，Nanjing210029，China Abstract Background：Lithium pretreatmenthasbeenprovedtobeneuro-protective．Post—ischem iaconditioningattractedmoreandmoreattentionduring thelastyears．Thisstudywasdesignedtoobservetheeffectoflithiumpost—treatmentonneuronapoptosisandglycogensynthasekinase(GSK)一313inrats aftermiddlecerebralarteryocclusion(MCAO)． M ethods：Therightmiddlecerebralarterywasoccludedwithanylonsuturefor90mins．RatswererandomlydividedintoNO group，SH group， IR group，Lilgroup (1mmol／kg)，Li2group (2mmol／kg)，andLi3group (3mmol／kg) ．Animalsineachgroupwerekilled6hours，1day， 3day,or7daysaftercerebralischemia，withsixratsineachtimepoint．NeuronapoptosisintheCA 1regionwereexaminedbyHoechststaining．The expressionanddistributionofGSK一313andP—GSK一313(Ser9)wasexaminedbyimmunofluorescenceanalysis． Results：TheapoptoticnucleiwasdetectedsignificantlyinIRgroupscomparedwithSHgroup(P0．01)，whichpeakedonedaylaterandthen declinedgraduallyinallgroupsComparedwithIRgroup，thenumberofapoptoticnucleireducedingroupstreatedwithlithiumchloridedosedependently( P001)． TherewasriovisibledifferenceinthetotalamountsofGSK一3betabetweenanyofthetwogroupsorbewt eenanyofthetwotimepointfP0．O11． TheamountsofP—GSK一3B (Ser9)seemedincreasedwiththetimeandtheywerehigherinIRgroupsthanthoseinSHgroups(PO．01)．Lithium post—treatmentreinforcedthistendencydose—dependently(PO．01)． Conclusion：Lithiumchloridecandose—dependentlyf1～3mmol／kg、suppressneuronapoptosisandGSK-313afterMCAO／reperfusioni~ury． Keywords：apoptosis；lithium chloride；glycogensynthasekinase一38：middlecerebralartery occlusion CorrespondingAuthor：Yan—ningQian，E—mail：yanningqian@yahoocom．Cll lNTRoDUCTIoN mightberelatedtothedown-·regulationofpro—a‘poptotic Lithium，amajordrugusedforbipol