丹参酮IIA对肝癌细胞血管内皮生长因子表达影响.docVIP

丹参酮IIA对肝癌细胞血管内皮生长因子表达的影响 　　作者：符寒，和水祥，徐俊丽，赵 刚，王艳丽，任牡丹 作者单位：西安交通大学医学院第一附属医院消化内科，陕西西安 710061 　　【摘要】 目的 观察丹参酮IIA对人肝癌SMMC7721细胞的增殖抑制和凋亡诱导作用，及对血管内皮生长因子(VEGF)表达的影响，探讨其可能的抗肿瘤作用机制。方法 体外培养人肝癌SMMC7721细胞株，MTT比色法观察细胞增殖抑制情况;荧光显微镜检测细胞凋亡;免疫细胞化学法和ELISA法分别检测细胞及细胞培养液中VEGF的表达情况。结果 丹参酮IIA对肝癌细胞的生长有明显的抑制作用，并呈剂量依赖性，以0.5mg/L终质量浓度的抑制作用最明显，其48h的抑制率为72.5%，与未加药对照组相比差异具有统计学意义(P0.01);荧光显微镜下经丹参酮IIA作用后的肝癌细胞可见凋亡小体形成;免疫细胞化学染色及ELISA法检测发现，丹参酮IIA作用组肝癌细胞VEGF表达和培养液中VEGF分泌量明显减少，与未加药对照组相比差异有统计学意义(P0.01)。结论 丹参酮IIA可下调细胞VEGF的表达，这可能是其抗肿瘤作用的机制之一。 　　【关键词】 丹参酮IIA;血管内皮生长因子(VEGF);肝癌;增殖抑制;凋亡 　　expression in hepatocellular carcinoma cell line SMMC7721FU Han, HE Shuixiang, XU Junli, ZHAO Gang, WANG Yanli, REN Mudan 　　(Department of Gastroenterology, the First Affiliated Hospital, 　　Medical School of Xian Jiaotong University, Xian 710061, China)ABSTRACT: Objective To evaluate the effects of Tanshinone IIA in inhibiting the proliferation, inducing apoptosis and the expression of vascular endothelial growth factor (VEGF) on SMMC7721 cell and to investigate the possible antitumor mechanism of Tanshinone IIA. Methods The human hepatocellular carcinoma cell line SMMC7721 was cultured in vitro. The cell growth activity was evaluated by MTT assay. The apoptosis of SMMC7721 was studied through the fluorescence microscope. The expression of VEGF of SMMC7721 cell and the cell culture fluid was determined by immunocytochemistry and ELISA assay, respectively. Results Tanshinone IIA treatment of SMMC7721 cells resulted in a strong growth inhibition in a dosedependent manner. The peak value of inhibition rate (72.5%) reached at 0.5mg/L Tanshinone IIA for 48h, and there were significant differences compared to control group (P0.01). The formation of apoptotic bodies of SMMC7721 cell was observed in Tanshinone IIAtreated group under the fluorescence microscope. The expression of VEGF in Tanshinone IIAtreated group was significantly lower than that in control group (P0.01). The similar result could