产弹性蛋白酶芽孢杆菌的筛选与鉴定.pdfVIP

25 3 Vol.25 No.3 2007 9 Journal of Sichuan Agricultural University Sep. 2007 Screening and Identif ication of Elastaseproduci ng Bacillus Strains 1 1 2 1 ZHA NG J uan , L I U S huliang , W U Q i , Z HA N L i ( 1.Lab of Farm Produce, Store Engineering in Sichuan Province, Sichuan Agricultural University, aan 625014, Sichuan, China; 2. College of Biology and Science, Sichuan Agricultural University, aan 625014, Sichuan, China) Abstract: From the soil and wastewater samples near the slaughterhouse, milk factory and beer facto ry, 209 strains with elastase activity were isolated. Furthermore, 83 strains were collected because of thehigher ratio of hydrolyzeloop and colony diameter (HC) . Finally, one strain with thehighest elas tase activity was screened and named strain SCEL01. Its elastase activity reached to 33u/ mL. T he i dentification showed that strain SCEL01 was Bacillus subtilis by the way of morphology, biochemistry characteristic and 16SrDNA sequence homology analysis. Key words: elastase; Bacillus; 16SrDNA; identification 1 1 2 1 张 娟 , 刘书亮 , 吴 琦 , 詹 莉 ( 1. 四川农业大学 农产品加工及贮藏工程省级重点实验室; 2. 四川农业大学 生命科学与理学院, 四川雅安 625014) : 从屠 场, 牛奶场和啤酒厂附近采集的土样和污水中, 通过弹性蛋白平板筛选获得209 株产弹性蛋白酶的细 菌菌株, 将弹性蛋白水解圈和菌落直径之比(HC) 比值较大的83 株菌通过摇瓶复筛后得到 一株酶活力较高的菌株 SCEL01, 产酶活性达到33u/mL结合形态学生理生化特性和16SrDNA 序列同源性分析, 该菌株被鉴定为枯草芽 孢杆菌( Bacillus subtilis) : 弹性蛋白酶; 芽孢杆菌; 16SrDNA; 鉴定 : Q939. 1 : A : 1000- 2650(2007) 03- 0253- 04 (Elastase) , [ 1] [ 5] ,,